大豆孢囊线虫侵染对乙烯合成及信号传导基因表达调控的研究  

Transcription of Ethylene Biosynthesis and Signaling Associated Genes in Response to Heterodera glycine Infection

作　　者：郭宾会 宋丽 GUO Bin-hui;SONG Li(Joint International Research Laboratory of Agriculture and Agri-Product Safety,Co-Innovation Center for Modern Production Technology of Grain Crops,Yangzhou University,Yangzhou 225009;Basic Experimental Teaching Center of Life Science,Yangzhou University,Yangzhou 225009)

机构地区：[1]扬州大学农业科技发展研究院教育部农业与农产品安全国际合作联合实验室,扬州225009 [2]扬州大学生命科学基础实验教学中心,扬州225009

出　　处：《生物技术通报》2022年第8期150-158,共9页Biotechnology Bulletin

基　　金：扬州市科技计划(政策引导类(国际科技合作)(YZ2018156))。

摘　　要：乙烯是一种重要的植物激素,其合成和信号传导相关基因参与调控植物生长发育及防御反应。为阐明大豆孢囊线虫侵染和定殖对寄主植物乙烯合成及信号传导途径的影响,本研究以8个抗病品种和2个感病品种为材料,利用荧光定量PCR方法对比接种与未接种孢囊线虫大豆根中乙烯合成基因GmACS和信号传导基因GmEIN的表达水平。实验结果表明,与感病品种相比,抗病品种中乙烯的生物合成受到显著调控,其中PI 548316中GmACS显著上调,而在PI 437654和PI 88788中GmACS显著下调。同时,GmEIN在4个抗病品种中轻微上调,但在其它抗病品种和2个感病品种中均未受到显著调控。研究表明GmACS和GmEIN的表达受到孢囊线虫侵染与定殖调控,其中被显著调控的GmACS基因可以作为提高大豆抗病育种的候选基因。Ethylene is an important plant hormone.Its synthesis and signal transduction related genes are involved in the regulation of plant growth,development and defense response.In order to clarify the effects of soybean cyst nematode infection and colonization on ethylene biosynthesis and signal transduction pathway,the expressions of ethylene synthesis gene GmACS and signal transduction gene GmEIN in soybean roots inoculated with and without cyst nematode were compared by fluorescence quantitative PCR.The results showed that compared with susceptible varieties,the ethylene biosynthesis in resistant varieties was significantly regulated,and in which 6 GmACSs genes were significantly induced in PI 548316,while significantly inhibited in PI 437654 and PI 88788.Meanwhile,several GmEINs was slightly upregulated in 4 resistant varieties,but not significantly regulated in other resistant varieties and two susceptible varieties.The results show that ethylene synthesis in some soybean varieties are significantly regulated by soybean cyst nematode infection and colonization,and the regulated genes can be used as candidates for improving soybean disease resistance breeding.

关 键 词：大豆 大豆孢囊线虫 GmACS基因 GmEIN基因 基因表达分析 

分 类 号：S435.651[农业科学—农业昆虫与害虫防治]