AOX基因家族的结构和功能特征分析  

Structural and Functional Characterization of AOX Gene Family

作　　者：王慧 马艺文[2] 乔正浩 常彦彩 术琨 丁海萍 聂永心[3] 潘光堂[1] WANG Hui;MA Yi-wen;QIAO Zheng-hao;CHANG Yan-cai;ZHU Kun;DING Hai-ping;NIE Yong-xin;PAN Guang-tang(Maize Research Institute of Sichuan Agricultural University,Chengdu 611130;Tonghua Academy Agricultural Sciences,Tonghua 135007;State Key Laboratory of Crop Biology,Shandong Agricultural University,Tai'an 271018)

机构地区：[1]四川农业大学玉米研究所,成都611130 [2]通化市农业科学研究院,通化135007 [3]山东农业大学作物生物学国家重点实验室,泰安271018

出　　处：《生物技术通报》2022年第7期160-170,共11页Biotechnology Bulletin

基　　金：国家自然科学基金面上项目(31771802)。

摘　　要：交替氧化酶(alternative oxidase,AOX)是植物呼吸途径上的一种末端氧化酶,通过对玉米、水稻、拟南芥、二穗短柄草、番茄、马铃薯、高粱、大豆等8个物种中AOX基因进行鉴定分析,以期进一步挖掘AOX基因的功能以及调控机制。在8个物种中共鉴定到35个编码AOX蛋白的基因,通过聚类分析将其划分为5个亚家族。通过分析和比较AOX基因的系统进化关系、染色体位置、外显子-内含子结构以及保守基序,进一步验证了该亚家族划分模式,同时也揭示了AOX基因在进化过程中具有较强的保守性。通过顺式作用元件预测、miRNA靶位点预测以及AOX蛋白的GO注释分析,发现AOX基因可能在植物生长发育以及应激反应中发挥重要作用。在玉米苗期进行低温、高温、干旱以及盐碱胁迫处理后,对玉米中4个AOX基因进行qRT-PCR分析表明,4个AOX基因呈现不同程度的诱导表达,暗示它们在逆境胁迫应答上存在密切关系。该研究结果为深入理解AOX基因的生物学功能以及响应逆境胁迫的调控机制奠定了基础。AOX(alternative oxidase,AOX)is a terminal oxidase in the respiratory pathway of plants.Through the identification and analysis of AOX genes in 8 species of Zea mays,Oryza sativa,Arabidopsis thaliana,Brachypodium distachyon,Solanum lycopersicum,Solanum tuberosum,Sorghum bicolor and Glycine max,we aim to further explore the function and regulatory mechanism of AOX gene.A total of 35 genes encoding AOX protein in these 8 species were identified,and these 35 genes were divided into 5 subfamilies by cluster analysis.The phylogenetic analysis and comparison of the chromosome position exon-intron structure and the conserved motif composition of AOX further supported the subfamily division model,and revealed that the AOX gene maintained a strong conserved nature during the evolution process.The prediction of cis-acting elements,miRNA target sites and the GO annotation analysis of AOX protein revealed that AOX gene may play an important role in plant growth and development and stress response.After stress treatment at the seedling stage of maize,the qRT-PCR analysis of 4 AOX genes in maize showed that the 4 AOX genes presented different degrees of inducible expression,suggesting that they were closely related in response to abiotic stress.These results lay a foundation for further understanding the biological function of AOX genes and their regulatory mechanism in response to abiotic stress.

关 键 词：AOX 基因家族鉴定 表达分析 非生物胁迫 

分 类 号：Q943.2[生物学—植物学]