两种细菌鉴定法在临床血液检验中的应用效果对比探讨  

Comparative Study on the Application Effect of Two Bacterial Identification Methods in Clinical Blood Test

作　　者：张玉启[1] ZHANG Yuqi(Department of Laboratory,Lanshan District People's Hospital,Linyi,Shandong Province,276000 China)

机构地区：[1]临沂市兰山区人民医院检验科,山东临沂276000

出　　处：《系统医学》2022年第14期75-78,共4页Systems Medicine

摘　　要：目的 分析在临床血液检验中采取直接药敏试验以及常规药敏试验的临床应用效果。方法 选取2019年1月—2020年1月临沂市兰山区人民医院收治的患者96例作为研究对象,所有患者均采集两份血液样本进行血液细菌检验,一份样本采取直接药敏试验法进行检验,另一份样本采取常规药敏试验法进行检验,并以细菌培养和检验结果作为金标准,比较直接药敏试验及常规药敏试验的检验合格率,并比较两种检验方法所需时间。结果 96例血液样本中经过细菌培养及检验共计检出阳性样本88例,使用常规药敏试验时检出阳性样本86例,检验合格率为97.73%,直接药敏试验检出阳性样本88例,检验合格率为100.00%。两种检验方法的检验合格率对比,差异无统计学意义(χ^(2)=2.023,P>0.05)。直接药敏试验法所用时间为(9.86±2.58)h,常规药敏试验法所用时间为(71.33±8.84)h,直接药敏试验法明显比常规药敏试验法所用时间要短,差异有统计学意义(t=65.402,P<0.05)。结论 在血液细菌检验时应用直接药敏试验和常规药敏试验均能够获得令人满意的检验结果,可以为临床用药进行指导,降低不合理用药的风险,对提高临床疗效及减少并发症发生风险均有非常重要的意义。Objective To analyze the clinical application effect of direct drug susceptibility test and routine drug susceptibility test in clinical blood test.Methods A total of 96 patients admitted to Lanshan District People’s Hospital from January 2019 to January 2020 were selected as the research subjects.Two blood samples were collected from all patients for blood bacteria tests.One sample was tested by direct drug susceptibility testing method,and the other sample was tested by conventional drug susceptibility testing method.Using bacterial culture and test results as the gold standard,the pass rates of direct drug susceptibility testing and conventional drug susceptibility testing were compared and the time required for the two testing methods was compared.Results Among the 96 blood samples,a total of 88 positive samples were detected by bacterial culture and testing,and 86 positive samples were detected by routine drug susceptibility testing.The test pass rate was 97.73%,88 positive samples were detected by direct drug susceptibility test,and the test pass rate was 100.00%.There was no significant difference in the test pass rate between the two methods(χ^(2)=2.023,P>0.05).The time used by the direct drug susceptibility test method was(9.86±2.58) h,and the time used by the conventional drug susceptibility test method was(71.33±8.84) h.It can be seen that the direct drug susceptibility test method takes significantly shorter time than the conventional drug susceptibility test method,the difference was statistically significant(t=65.402,P<0.05).Conclusion The application of direct drug susceptibility test and routine drug susceptibility test in blood bacteria test can obtain satisfactory test results.It can guide clinical medication and reduce the risk of irrational medication,which is of great significance for improving clinical efficacy and reducing the risk of complications.

关 键 词：直接药敏试验 常规药敏试验 细菌鉴定 血液样本 细菌培养 

分 类 号：R446.1[医药卫生—诊断学]