棉花GhGGB基因互补表达载体的构建及其抗旱性分析  

Complementary Expression Vector Construction and Drought Resistance Analysis of GhGGB Gene in Cotton(Gossypium hirsutum)

作　　者：胡子曜 阿尔祖古丽·塔什 雷建峰[2] 代培红[1] 柳建飞 邓嘉辉 刘超[1] 刘晓东[1] 李月[1] HU Zi-Yao;AERZUGULI·Ta-Shi;LEI Jian-Feng;DAI Pei-Hong;LIU Jian-Fei;DENG Jia-Hui;LIU Chao;LIU Xiao-Dong;LI Yue(College of Life Sciences,Xinjiang Agricultural University,Urumqi 830052,China;College of Agronomy,Xinjiang Agricultural University,Urumqi 830052,China)

机构地区：[1]新疆农业大学生命科学学院,乌鲁木齐830052 [2]新疆农业大学农学院,乌鲁木齐830052

出　　处：《农业生物技术学报》2022年第9期1687-1697,共11页Journal of Agricultural Biotechnology

基　　金：国家自然科学基金(31560534)。

摘　　要：GGB(geranyl geranyltransferase beta)基因编码Ⅰ型蛋白香叶酰基转移酶,参与蛋白异戊二烯化修饰,在植物响应逆境胁迫过程中具有重要的调控作用。为探究棉花(Gossypium hirsutum)GhGGB基因的生物学功能,本研究构建植物互补表达载体pBI101-AtGGBP-GhGGB-AtGGBT并转化拟南芥(Arabidopsis thaliana)ggb突变体,筛选获得GhGGB转基因株系。检测自然干旱胁迫下GhGGB转基因株系、拟南芥ggb突变体和野生型拟南芥Col-0的表型和生理生化指标。结果显示,以野生型拟南芥Col-0为对照,转基因GhGGB植株对干旱胁迫的敏感性显著增强,而ggb突变体植株则表现出了更强的抗旱性,各组分生理指标的测定结果与表型一致,表明GhGGB基因互补了拟南芥ggb突变体的功能缺失,降低了耐旱性。本研究结果初步表明GhGGB是一个抗旱负调控基因,为棉花抗旱种质培育提供新的基因选择。GGB(geranyl geranyltransferase beta)gene encodes typeⅠprotein geranyltransferase,which is involved in protein isoprene modification and plays an important regulatory role in plant response to stress.In order to explore the biological function of GhGGB gene in cotton(Gossypium hirsutum),the plant complementary expression vector pBI101-AtGGBP-GhGGB-AtGGBT was constructed and transformed into ggb mutant of Arabidopsis thaliana,and GhGGB transgenic lines were screened.Phenotypic,physiological and biochemical indexes of GhGGB transgenic lines,ggb mutant and wild type Arabidopsis Col-0 under natural drought stress were detected.The results showed that with wild-type Arabidopsis Col-0 as the control,the sensitivity of transgenic GhGGB plants to drought stress was significantly enhanced,while the ggb mutant plants showed stronger drought resistance.The determination results of physiological indexes of each component were consistent with the phenotype,indicating that GhGGB gene complemented the functional deficiency of ggb mutant in Arabidopsis and reduced drought tolerance.The results of this study preliminarily showed that GhGGB was a negative regulatory gene for drought resistance,which provides a new gene selection for cotton drought-resistant germplasm cultivation.

关 键 词：棉花 GhGGB 互补表达 干旱胁迫 生理生化指标 

分 类 号：S188[农业科学—农业基础科学]