葡萄G2-like转录因子家族的鉴定及在穗梗褐变过程中的表达分析  

Characterization of the G2-like Transcription Factor Family of Grape(Vitis vinifra)and Its Expression Analysis in Rachis Browning

作　　者：李志谦 邹东方 李靖雯 叶霞[1] 冯建灿[1] LI Zhi-Qian;ZOU Dong-Fang;LI Jing-Wen;YE Xia;FENG Jian-Can(College of Horticulture,Henan Agriculture University,Zhengzhou 450002,China)

机构地区：[1]河南农业大学园艺学院,郑州450002

出　　处：《农业生物技术学报》2022年第9期1713-1723,共11页Journal of Agricultural Biotechnology

基　　金：国家自然科学基金(32002017)。

摘　　要：Golden2-like(G2-like)转录因子在植物叶绿体发育、叶绿素积累、光合作用和衰老过程中发挥着重要作用。为探究G2-like转录因子在葡萄(Vitis vinifra)生长发育过程中的功能,本研究利用生物信息学方法鉴定了葡萄中G2-like转录因子家族,在聚类、保守结构域和共线性分析基础上,对其在葡萄不同组织及穗梗褐变过程中的表达量进行了测定。结果显示,在葡萄基因组中共鉴定到43个G2-like转录因子基因,分为6个亚家族,不均匀地分布在葡萄的17条染色体上。葡萄和拟南芥(Arabidopsis thaliana)中存在34对同线基因,5个片段复制和1个随机复制,且存在Myb DNA-binding保守结构域。q PCR结果显示,在葡萄穗梗褐变过程中,VvG1、VvG28、VvG43、VvG2、VvG14、VvG6和VvG24基因的表达与叶绿素含量的变化呈正相关,而VvG27基因的表达与叶绿素含量的变化呈负相关。研究结果表明,G2-like基因可能参与葡萄的器官发育和穗梗褐变过程。本研究为进一步探讨研究G2-like基因功能提供了参考依据。Golden2-like(G2-like)transcription factors play important roles during chloroplast development,chlorophyll accumulation,photosynthesis and senescence in plants.In order to explore the role of G2-like transcription factor during grape(Vitis vinifra)development,bioinformatics method was used to identifiy G2-like family transcription factor in grape,on the basis of phylogenetic,conserved domain and synteny analysis,expression of G2-like in different organs and during rachis browning was quantified.A total of 43 G2-like(VvG1~VvG43)transcription factors uneven distributed in 17 chromosomes were identified in grape genome,and were classified into 6 groups based on phylogeny analysis with Arabidopsis thaliana.34 pairs of synteny between A.thaliana and grape,5 segmental duplication,1 tandem duplication and conserved Myb DNAbinding domain were found in grape.qPCR results revealed that VvG1,VvG28,VvG43,VvG2,VvG14,VvG6,VvG24 showed positive correlation with chlorophyll content variation during rachis browning of grape,but VvG27 showed negative expression pattern.This results indicated G2-like genes played the important role in tissue development and rachis browning of grape,which provides a reference basis for further function analysis of G2-like genes.

关 键 词：葡萄 Golden2-like(G2-like) 生物信息学 器官 穗梗褐变 

分 类 号：S663.1[农业科学—果树学]