基于转录组测序的ITGAD基因InDel位点鉴定及其与山羊生长性状的关联性分析  

Identification of InDel Locus of ITGAD Gene Based on Transcriptome Sequencing and Its Association with Goat(Capra hircus)Growth Traits

作　　者：吴贤锋 王金宝[2] 刘远[1] 池春梅[2] 黄勤楼[1] 李文杨[1] WU Xian-Feng;WANG Jin-Bao;LIU Yuan;CHI Chun-Mei;HUANG Qin-Lou;LI Wen-Yang(Institute of Animal Husbandry and Veterinary,Fujian Academy of Agricultural Sciences,Fuzhou 350000,China;Ningde Institute of Agricultural Science,Fuan 355017,China)

机构地区：[1]福建省农业科学院畜牧兽医研究所,福州350000 [2]宁德市农业科学研究所,福安355017

出　　处：《农业生物技术学报》2022年第9期1763-1770,共8页Journal of Agricultural Biotechnology

基　　金：福建省农业科学院探索类项目(ZYTS2019020);福建省省属公益类科研院所基本科研专项(2020R10260012,2020R10260014,2019R1026-10)。

摘　　要：2整合素αD(β2 integrinsαD,ITGAD)是一种异二聚体细胞表面受体的亚基,在调控脂肪新陈代谢和血糖平衡中发挥重要作用。为了加快山羊(Capra hircus)生长性状选育的遗传进展,为山羊生长性状的标记辅助选择(marker assisted selection,MAS)提供理论依据,本研究利用DNA池技术和高分辨率熔解曲线(high resolution melting,HRM)分析技术,检测了ITGAD基因在3个山羊品种(共355只)的插入/缺失(Insertion/Deletion,InDel)多态性,并分析其与山羊生长性状之间的关联效应。结果表明,检测到1个山羊ITGAD基因第15个内含子的新InDel位点(NC_030832.1:g.15243_15244 ins C),其多态信息含量(PIC)为0.289~0.369,并且该位点在3个品种中都处于哈迪-温伯格平衡状态;在与生长性状的关联分析中,该位点与福清山羊的尻宽和简阳大耳羊的体高、体长和胸围均显著相关(P<0.05),与简阳大耳羊的胸深、胸宽、管围、尻宽和管围指数极显著相关(P<0.01),并且II基因型个体显著优于DD基因型。本研究发现ITGAD基因InDel位点与山羊生长性状有显著的关联效应,为山羊生产性状的分子遗传标记选择提供参考依据。2 integrinsαD(ITGAD)is a subunit of heterodimeric surface receptor,and plays a critical role in the regulation of lipid metabolism and blood glucose balance.This work aimed to detect the candidate molecular markers for marker-assisted selection(MAS)in goats(Capra hircus).In this study,the Insertion/Deletion(InDel)polymorphism of ITGAD gene in 3 goat breeds(355 in total)was identified through DNA pooling technology and high-resolution melting(HRM)analysis.The results showed that a novel InDel locus(NC_030832.1:g.15243_15244 ins C)was detected.Furthermore,in 3 goat breeds,the polymorphic information content(PIC)of the InDel locus was 0.289~0.369.The InDel locus was consistent with HardyWeinberg in 3 experimental populations.Association analysis showed that this InDel locus was significantly associated with the huckle bone width of Fuqing goat and the body height,body length,and chest circumference of Jianyang Daer goat(P<0.05).It was also extremely significantly correlated with chest depth,chest width,cannon circumference,and cannon circumference index of Jianyang Daer goat(P<0.01).Moreover,the II genotype conferred positive effects on growth traits compared with the DD genotype.The present study found the significant association of ITGAD InDel locus with the growth traits in goats,which could be adopted as the molecular genetic marker for growth traits of goats.

关 键 词：山羊 β2整合素αD(ITGAD)基因 插入缺失(InDel) 高分辨率熔解曲线(HRM) 生长性状 

分 类 号：S828.2[农业科学—畜牧学]