基因编辑在家禽转基因研究中的应用进展  

Progress of Gene Editing and Its Application in Transgenic Poultry

作　　者：张宛玉 郑喜邦[1] ZHANG Wan-Yu;ZHENG Xi-Bang(College of Animal Science and Technology,Guangxi University,Nanning 530004,China)

机构地区：[1]广西大学动物科学技术学院,南宁530004

出　　处：《农业生物技术学报》2022年第9期1834-1844,共11页Journal of Agricultural Biotechnology

基　　金：国家自然科学基金(32060738,31660653);广西自然科学基金重点项目(2018GXNSFDA281026);广西科技计划重点研发项目(桂科AB16380098)。

摘　　要：家禽是重要的模式生物,在农业和生物医学研究领域发挥重要作用。近年来基因编辑工具TALEN和CRISPR/Cas9在家禽转基因领域得以成功应用。这些基因编辑技术可望用于提高家禽生产性能、研究病毒与受体之间的相互作用、研发病毒载体疫苗、改善动物福利、改善禽蛋消费的安全性、在卵白中生产药用蛋白和抗体等。本文综述了家禽基因编辑的途径及CRISPR/Cas9在家禽生产与育种以及基础研究领域的应用现状,并展望了未来家禽基因编辑的应用前景。本综述对利用基因编辑手段提高家禽生产性能和预防禽类病毒性疾病具有指导意义。Avian is a valuable animal model,and plays a significant part in agriculture and biomedicine.In recent years,newly developed programmable gene editing tools such as TALEN and CRISPR/Cas9 have been successfully adopted in avian species.These gene modification technologies are expected to be applied to practical uses,such as enhancing economic traits of poultry,investigating virus-receptor interaction,developing vector vaccines,improving poultry welfare,and producing pharmaceutical proteins and antibodies in eggs,etc.In this review,we present a concise description of the existing approaches of genome editing in bird species,and the application of CRISPR/Cas9 in advancing poultry breeding and production,as well as in poultry research.In addition,we discuss the potential use of gene-edited poultry model in various industries in the future.This review will lead to improve economic traits of poultry and control avian viral diseases by means of gene editing techniques.

关 键 词：家禽 基因编辑 CRISPR/Cas9 TALEN 原始生殖细胞(PGCs) 

分 类 号：S-83[农业科学]