孕中期丙泊酚麻醉对子代认知和海马miRNAs的影响  

Effects of propofol anesthesia in the second trimester on offspring cognition and hippocampal miRNAs

作　　者：赵黎丽[1] 罗佛全 龚海霞[1] 刘添银[1] 曹蒙蒙 ZHAO Lili;LUO Foquan;GONG Haixia;LIU Tianyin;CAO Mengmeng(Department of Anesthesiology,the First Affiliated Hospital of Nanchang University,Nanchang,Jiangxi 330006,China;Department of Anesthesiology,Zhejiang Provincial People′s Hospital,Hangzhou,Zhejiang 310000,China;Department of Anesthesiology,Jiujiang First People′s Hospital,Jiujiang,Jiangxi 332000,China)

机构地区：[1]南昌大学第一附属医院麻醉科,江西南昌330006 [2]浙江省人民医院麻醉科,浙江杭州310000 [3]江西省九江市第一人民医院麻醉科,江西九江332000

出　　处：《检验医学与临床》2022年第17期2341-2343,2348,共4页Laboratory Medicine and Clinic

基　　金：江西省卫生健康委科技计划项目(20185106)。

摘　　要：目的探讨孕中期丙泊酚麻醉对子代认知、海马microRNAs(miRNAs)表达的影响。方法将孕14 d的SD大鼠随机分为丙泊酚组(P组)和脂肪乳剂对照组(C组)。P组接受丙泊酚麻醉4 h,C组给予20%脂肪乳剂。子鼠进行悬崖回避实验、Morris水迷宫(MWM)实验、海马miRNAs芯片检测。结果与C组比较,P组子鼠的悬崖回避时间延长,MWM实验中第4天和第5天的逃避潜伏期延长、平台穿越次数减少(P<0.05);出生后7 d和出生后30 d的子鼠海马miRNAs芯片检测结果发现,P组相比C组分别有9个和3个差异表达的miRNAs(P<0.05)。结论孕中期丙泊酚麻醉损伤子代认知功能,影响海马miRNAs表达。Objective To investigate the effects of propofol anesthesia in the second trimester of pregnancy on offspring cognition and expression of microRNAs(miRNAs)in hippocampus.Methods SD rats at 14 days of gestation were randomly divided into a propofol group(P group)and a fat emulsion control group(C group).Group P received propofol anesthesia for 4 hours,and group C received 20%fat emulsion.The mice were tested by cliff avoidance experiment,Morris water maze(MWM)experiment,and hippocampal miRNAs chip detection.Results Compared with the C group,the cliff avoidance time of the mice in the P group was prolonged,the escape latency on the 4th and 5th days in the MWM experiment was prolonged,and the number of platform crossings was decreased(P<0.05).The results of microarray detection of miRNAs in the hippocampus of offspring mice at postnatal 7 d and postnatal 30 d showed that there were 9 and 3 differentially expressed miRNAs in group P compared with group C,respectively(P<0.05).Conclusion Propofol anesthesia in the second trimester impairs the cognitive function of offspring and affects the expression of miRNAs in the hippocampus.

关 键 词：孕中期 丙泊酚 子代 认知 MICRORNAS 

分 类 号：R614.2[医药卫生—麻醉学]