CLDN1与P16、Ki67在宫颈上皮内病变组织中的表达比较及意义  

Expression Comparison and Significance of CLDN1,P16 and Ki67 in Cervical Intraepithelial Lesions

作　　者：王琳[1] 陈九伟 吕秀萍[2] WANG Lin;CHEN Jiuwei;LYU Xiuping(Department of Gynecology,Zibo Maternal and Child Health Hospital,Zibo,Shandong Province,255000 China;Department of Gynecology,Affiliated Hospital of Weifang Medical College,Weifang,Shandong Province,261000 China)

机构地区：[1]淄博市妇幼保健院妇科,山东淄博255000 [2]潍坊医学院附属医院妇科,山东潍坊261000

出　　处：《系统医学》2022年第13期45-50,共6页Systems Medicine

摘　　要：目的比较CLDN1、P16、Ki673种生物标记物在子宫颈上皮内瘤变(cervical intraepithelial neoplasia,CIN)1~3级组织中的阳性表达,并探讨其意义。方法选取淄博市妇幼保健院在2016年1月—2017年12月经病理诊断为CIN的宫颈组织石蜡包埋标本62例,其中CIN1级22例,CIN2级18例,CIN3级22例,采用SP法分别进行P16、Ki67和CLDN1的免疫组化染色并检测其阳性表达。结果P16在CIN1~3级中的阳性表达率分别为45.5%、61.1%、100.0%,差异有统计学意义(χ^(2)=16.209,P<0.001),Ki67在CIN1~3级组织中的阳性表达率分别为50.0%、55.6%、100.0%,差异有统计学意义(χ^(2)=15.211,P<0.001),P16、Ki67在CIN1~3级组织中的阳性表达率均是逐渐增加的,二者的阳性率均与CIN级别呈正相关关系(r1=0.658,r2=0.604);CLDN1的阳性表达率并不随病变级别的增加而增加(68.2%、11.1%、36.4%),与CIN病变级别的增加无明显相关关系(r=-0.340)。P16与Ki67的阳性表达具有相关性,CLDN1与P16、Ki67的阳性表达均没有相关性。结论P16、Ki67可以作为CIN分级诊断的辅助指标,提高诊断的准确性和一致性,为临床治疗及预后提供有效信息;CLDN1在CIN进展中的作用及病理诊断意义尚不明确,还需要进一步的研究和探讨。Objective To compare the positive expressions of CLDN1,P16 and Ki67 biomarkers in cervical intraepithelial lesion(CIN)grade 1~3 tissues,and to explore their significance.Methods A total of 62 cervical tissue paraffinembedded specimens with pathological diagnosis of CIN in Zibo Maternal and Child Health Hospital from January2016 to December 2017 were selected,including 22 cases of CIN1 grade,18 cases of CIN2 grade,and 22 cases of CIN3 grade.The immunohistochemical staining of P16,Ki67 and CLDN1 was performed by SP method and their positive expressions were detected.Results The positive expression rates of P16 in CIN1-3 were 45.5%,61.1%and100.0%respectively,the difference was statistically significant(χ^(2)=16.209,P<0.001).The positive expression rates of Ki67 in CIN1-3 tissues were 50.0%,55.6%and 100.0%respectively,the difference was statistically significant(X2=15.211,P<0.001).The positive expression rates of P16 and Ki67 in CIN grades 1 to 3 were gradually increased,and the positive rates of both were positively correlated with CIN grades(r=0.658,r=0.604).The positive expression rate of CLDN1 did not increase with the increase of lesion grade(68.2%,11.1%,36.4%),and had no significant correlation with the increase of CIN lesion grade(r=-0.340).The positive expressions of P16 and Ki67 were correlated,but CLDN1 had no correlation with the positive expressions of P16 and Ki67.Conclusion P16 and Ki67 can be used as auxiliary indicators for CIN grading diagnosis,improve the accuracy and consistency of diagnosis,and provide effective information for clinical treatment and prognosis.The role of CLDN1 in the progression of CIN and the significance of pathological diagnosis are still unclear,and further research and exploration are needed.

关 键 词：CLDN1 P16 KI67 宫颈上皮内病变 免疫组化 

分 类 号：R4[医药卫生—临床医学]