血管软化丸抑制血管炎症反应防治动脉粥样硬化的分子机制  

Molecular mechanism of Xueguan Ruanhua Pill in preventing atherosclerosis through inhibiting vascular inflammation

作　　者：秦合伟[1] 牛雨晴 宋雪梅 王梦楠 孙孟艳 QIN Hewei;NIU Yuqing;SONG Xuemei;WANG Mengnan;SUN Mengyan(The Second Affiliated Hospital of He′nan University of Chinese Medicine,Zhengzhou 450002,China)

机构地区：[1]河南中医药大学第二附属医院康复科,郑州450002

出　　处：《实用医学杂志》2022年第15期1901-1907,共7页The Journal of Practical Medicine

基　　金：国家自然科学基金(编号:81704030);河南省中医药科学研究专项重点课题(编号:20-21ZY1023);中原英才计划中原青年拔尖人才项目(编号:豫组通[2021]44号);河南省科技攻关计划项目(编号:212102310359);河南省中医药拔尖人才培养项目资助(编号:豫卫中医函[2021]15号)。

摘　　要：目的观察中药复方血管软化丸防治动脉粥样硬化(atherosclerosis,AS)是否通过靶向调控长链非编码RNA-TGFB2-OT1、miR4459及其下游信号抑制炎症反应产生效果。方法建立AS模型,采用血管软化丸干预、以TGFB2-OT1 inhibitor作为阳性对照;干预8周后进行生化检测,HE染色观察主动脉病理改变,Real-time PCR法和Western blot法检测主动脉TGFB2-OT1、miRNA4459、LARP1、SQSTM1、CASP1、IL1B水平。结果与模型组相比,血管软化丸组TC、TG和LDL-C水平降低(均P<0.05),主动脉LA较大、IMT较薄、PA较小、LCA较小(P<0.05),主动脉TGFB2-OT1光密度值和荧光强度较低(均P<0.05),血清ICAM-1、VCAM-1、IL-8和MCP-1水平较低(均P<0.05),主动脉TGFB2-OT1、miRNA4459、LARP1、SQSTM1水平较低(均P<0.05),主动脉TGFB2-OT1、CASP1、IL1B蛋白表达水平较低(均P<0.05)。结论血管软化丸防治AS的分子机制与靶向调控长链非编码RNA-TGFB2-OT1、miR4459及其下游信号抑制炎症反应有关。Objective To observe whether the Chinese medicine Xueguan Ruanhua Pill can prevent and treat atherosclerosis(AS)by targeting the regulation of long-chain non-coding RNA-TGFB2-OT1,miR4459 and their downstream signals to inhibit inflammatory response.Methods AS model was established;Xueguan Ruanhua Pill was used for intervention,and TGFB2-OT1 inhibitor was used as a positive control.After 8 weeks of intervention,biochemical detection was performed,HE staining was used to observe the pathological changes of the aorta,and real-time PCR and western-blot were used to detect aortic TGFB2-OT1,miRNA4459,LARP1,SQSTM1,CASP1,and IL1B level.Results Compared with the model group,the level of TC,TG and LDL-C in Fufang Xueguan Ruanhua Pill group decreased(all P<0.05);larger aortic LA,thinner IMT,and smaller PA and LCA were found(all P<0.05).The optical density and fluorescence intensity of aortic TGFB2-OT1 were lower(all P<0.05),and the level of serum ICAM-1,VCAM-1,IL-8 and MCP-1 was lower(all P<0.05).The level of OT1,miRNA4459,LARP1,and SQSTM1 was reduced(all P<0.05),and the protein expression level of TGFB2-OT1,CASP1,and IL1B in the aorta was lower(all P<0.05).Conclusion The molecular mechanism of Xueguan Ruanhua Pill in the prevention and treatment of AS is related to the targeted regulation of long-chain non-coding RNA-TGFB2-OT1,miR4459 and their downstream signals to inhibit the inflammatory response.

关 键 词：动脉粥样硬化 长链非编码RNA-TGFB2-OT1 血管软化丸 炎症反应 

分 类 号：R245.97[医药卫生—针灸推拿学]