理脾复方制剂对厌食症大鼠下丘脑脑肠肽的影响  

Effects of Li-pi compound preparation on brain-gut peptide of hypothalamus in anorexic rats

作　　者：刘启艳 高娜 张力文 刘雅恬 庞平 詹伟 彭玉 LIU Qiyan;GAO Na;ZHANG Liwen;LIU Yatian;PANG Ping;ZHAN Wei;PENG Yu(Department of Pediatric Rehabilitation Medicine,Guiyang Maternal and Child Health Hospital,Guiyang,Guizhou,550003,China;Department of Pediatrics,Tongren Municipal Hospital of Traditional Chinese Medicine,Tongren,Guizhou,554300,China;Department of Pediatrics,the Second Affiliated Hospital of Guizhou University of Traditional Chinese Medicine,Guiyang,Guizhou,550003,China;School of Postgraduate,Guizhou University of Traditional Chinese Medicine,Guiyang,Guizhou,550002,China;Department of Pediatrics,Bijie Municipal Hospital of Traditional Chinese Medicine,Bijie,Guizhou,551702,China)

机构地区：[1]贵阳市妇幼保健院儿童康复医学科,贵州贵阳550003 [2]铜仁市中医医院儿科,贵州铜仁554300 [3]贵州中医药大学第二附属医院儿科,贵州贵阳550003 [4]贵州中医药大学研究生院,贵州贵阳550002 [5]毕节市中医院儿科,贵州毕节551702

出　　处：《中医儿科杂志》2022年第4期11-17,共7页Journal of Pediatrics of Traditional Chinese Medicine

基　　金：国家自然科学基金地区项目(81660808)。

摘　　要：目的 探讨理脾复方制剂治疗小儿厌食症的作用机制及靶点。方法 50只SPF级SD大鼠,适应性喂养3 d后按照随机数字表法分为空白组(N组)10只和模型组(M组)40只。N组给予常规饲料喂养,M组给予厌食症模型饲料喂养,造模成功后将M组再次按照随机数字表法分成4组,即模型对照组(MC组),理脾复方制剂低、中、高剂量组(LD、D、HD组),每组10只。N组、MC组给予饮用水,LD组、D组和HD组分别给予理脾复方制剂,每日灌胃1次,7 d后禁食24 h,观察每组的摄食量、体质量及下丘脑瘦素(LP)、神经肽Y(NPY)、八肽胆囊收缩素(CCK-8)蛋白浓度、蛋白与mRNA相对表达量。结果 大鼠摄入厌食症模型饲料第5天后摄食量及体质量分别减少48.1%和14.4%,提示造模成功。理脾复方制剂干预后,各组大鼠摄食量均明显增加,HD组>N组>D组>LD组>MC组,其中LD组和D组大鼠摄食量呈逐日增加趋势,HD组摄食量较为稳定;MC组于第3天逐渐增加,但均低于其余各组。各组大鼠每日体质量均明显低于N组,差异均有统计学意义(P<0.01);与MC组比较,LD组和D组体质量增长不明显,差异无统计学意义(P>0.05),HD组于第3天体质量明显增加,差异有统计学意义(P<0.05),第6天增加更显著(P<0.01)。与N组比较,其余各组下丘脑组织中LP、NPY、CCK-8蛋白浓度明显降低,差异均有统计学意义(P<0.01);与MC组比较,LD组、D组及HD组可显著上调下丘脑中LP、NPY、CCK-8蛋白浓度(P<0.01);LD组、D组及HD组两两比较,LP、NPY、CCK-8蛋白浓度差异均有统计学意义(P<0.01),其上调作用与剂量呈正相关,均以高剂量调节作用最为明显。与N组比较,MC组LP蛋白相对表达量增高(P<0.05),NPY与CCK-8蛋白相对表达量下降(P<0.05);与MC组比较,LD组、D组及HD组LP蛋白相对表达量均明显下降,LD组和HD组NPY、CCK-8蛋白相对表达量均明显上升,差异均有统计学意义(P<0.05);LD组、D组及HD组间LP蛋白相对表达量比较,差异�Objective To explore the mechanism and targets of Li-pi compound preparation in the treatment of anorexic rats.Methods After 3 days of adaptive feeding,50 SPF SD rats were divided into blank group(N group) with 10 rats and model group(M group) with 40 ones according to random number table method.Group N was fed with conventional feeds and group M was fed with anorexic model feeds;After successfully establishing modeling,M group was divided into 4 groups again according to random number table method,namely model control group(MC group) and low,medium and high dose groups of Li-pi compound preparation(LD,D and HD groups),with 10 rats in each group;N group and MC group were given drinking water,LD group,D group and HD group were given Li-pi compound preparation by gavage once a day,respectively,and fasted for 24 hours after 7 days.The effects of food intake,body mass,hypothalamic leptin(LP),neuropeptide Y(NPY),octapeptide cholecystokinin(CCK-8) protein concentration and relative expression of protein and mRNA were observed.Results After 5 days of ingestion of anorexia model feeds,the food intake and body mass of rats decreased by 48.1% and 14.4%,respectively,indicating that the model was successfully established.After the intervention of Li-pi compound preparation,the food intake of rats in each group increased significantly,HD group>N group>D group>LD group>MC group.The food intake thereof in LD group and D group increased day by day,and the food intake of HD group was relatively stable;That of MC group increased gradually on the 3 rd day,but lower than other groups.The daily body mass of rats in each group was significantly lower than that in N group,and the difference was statistically significant(P<0.01);The increase of body mass in LD group and D group was not significant as compared with MC group,and the difference was not statistically significant(P>0.05).The body mass of HD group increased significantly on the 3 rd day,and the difference was statistically significant(P<0.05),and the increase was more signifi

关 键 词：厌食症 大鼠 理脾复方制剂 瘦素 神经肽丫 八肽胆囊收缩素 实验研究 

分 类 号：R285.5[医药卫生—中药学]