一种快速识别苯硫酚的荧光探针的构建及其在细胞和环境样品中的应用  

Construction of a fluorescent probe for rapid recognition of thiophenol and its application in living cells and environmental samples

作　　者：王芹[1] 刘智峰[1] 李利华[1] 黄佩 赵佐平[1] 卢久富[1] 张晟瑞[1] WANG Qin;LIU Zhifeng;LI Lihua;HUANG Pei;ZHAO Zuoping;LU Jiufu;ZHANG Shengrui(Shaanxi Key Laboratory of Catalysis,School of Chemistry and Environment Science,Shaanxi University of Technology,Hanzhong,723000,China)

机构地区：[1]陕西省催化基础与应用重点实验室陕西理工大学化学与环境科学学院,汉中723000

出　　处：《环境化学》2022年第8期2675-2683,共9页Environmental Chemistry

基　　金：国家自然科学基金(22177066);陕西省创新人才推进计划-青年科技新星项目(2021KJXX-51);陕西省自然科学基础研究计划(2021JQ-751,2020JM-598);秦巴生物资源与生态环境国家重点实验室(培育)“市校共建”科研专项(SXJ-2103,SXJ-2105)资助。

摘　　要：由于苯硫酚(thiophenol,PhSH)在环境及生物体系中的剧毒性,开发可特异性识别PhSH的探针具有重要意义.本研究以香豆素为荧光团,2,4-二硝基苯磺酰胺为识别单元,基于分子内光诱导电荷转移机制,开发了一种新型的荧光增强型探针CPH,实现PhSH的检测.该探针能高选择性、高灵敏度地区分生物硫醇和PhSH,具有细胞渗透性好、毒性低、响应快的特点.值得注意地是,探针CPH可对不同浓度的PhSH迅速做出反应,具有良好的定量检测PhSH的能力,线性响应范围为0—16μmol·L^(-1),检出限为0.13μmol·L^(-1).此外,新型探针可成功检测HeLa细胞中的PhSH,且可定量测定水样中PhSH的含量,回收率可达95%以上.实验结果表明探针在环境科学和生物科学领域具有广阔的应用前景.Due to the extreme toxicity of thiophenol in environmental and biological systems,it is of great significance to develop a probe for the specific recognition of thiophenol.In this study,a novel off-on fluorescent probe CPH was developed using coumarin as the fluorophore and 2,4-dinitrobenzene sulfonate as the recognition unit based on intramolecular photo-induced charge transfer mechanism.This probe exhibiting good cell permeability,low toxicity and fast response can distinguish thiophenol from biothiols with high selectivity and sensitivity.It is noteworthy that the probe CPH can quickly respond to different concentrations of thiophenol,and a good linear relationship between the fluorescence intensity of CPH and thiophenol concentration(0—16μmol·L^(-1))was performed,displaying a limit of detection of 0.13μmol·L^(-1).In addition,the new probe has been successfully used for determining thiophenol in He La cells and environmental water samples with a recovery of more than 95%,indicating that the probe CPH has a broad application prospect in environmental science and biological science.

关 键 词：荧光探针 苯硫酚 高选择性 细胞成像 环境水样 

分 类 号：X832[环境科学与工程—环境工程] O657.3[理学—分析化学]