芒果MiNAC1基因的功能研究  

Functional Analysis of a MiNAC1 Gene in Mango

作　　者：杨小州 周少丽 何新华[1] 刘源 余海霞[1] 陆婷婷 王卓 罗聪[1] YANG Xiaozhou;ZHOU Shaoli;HE Xinhua;LIU Yuan;YU Haixia;LU Tingting;WANG Zhuo;LUO Cong(State Key Laboratory for Conservation and Utilization of Subtropical Agro-Bioresources/National Demonstration Center for Experimental Plant Science Education/College of Agriculture,Guangxi University,Nanning,Guangxi 530004,China)

机构地区：[1]亚热带农业生物资源保护与利用国家重点实验室/植物科学国家级实验教学示范中心/广西大学农学院,广西南宁530004

出　　处：《热带作物学报》2022年第8期1527-1534,共8页Chinese Journal of Tropical Crops

基　　金：国家现代农业产业技术体系广西芒果创新团队栽培与病虫害防治岗位项目(No.nycytxgxcxtd-2021-06-2);广西自然科学基金项目(No.2014GXNSFBA118102);科技先锋队‘强农富农’‘六个一’专项行动(No.202204)。

摘　　要：NAC转录因子在植物非生物逆境胁迫应答中发挥重要作用,研究芒果MiNAC1基因的功能为芒果的抗逆性育种提供基因资源。干旱、盐和低温等非生物胁迫严重影响芒果的生长发育。前期研究中,课题组从芒果逆境胁迫转录组中获得了一个MiNAC1基因,表达模式分析发现其与芒果的逆境胁迫应答有关。本研究对芒果MiNAC1基因的功能进行了验证。将芒果MiNAC1基因构建到pBI121-MiNAC1超量表达载体中,并利用农杆菌介导的花序浸染法转化野生型拟南芥,对获得的T3代纯合株系进行表型观察分析和逆境胁迫处理。结果显示,转芒果MiNAC1基因与野生型拟南芥的表型类似,转基因不影响拟南芥的莲座叶数量、抽薹时间、开花时间以及开花时的植株高度。逆境胁迫处理:分别用0、200、300、400mmol/L甘露醇进行干旱胁迫;用0、100、150、200mmol/LNaCl进行盐胁迫;4℃低温胁迫处理转基因与对照拟南芥植株。结果显示:随着甘露醇处理浓度的增加,转基因株系与对照组拟南芥的根系生长发育均受到抑制,但转基因株系受到抑制的影响显著小于对照组拟南芥,比如在300 mmol/L甘露醇处理时,转基因株系的根长显著增长,OE9的根长是WT的1.51倍,侧根数量显著增加,OE7的侧根数是WT的5倍,另外根冠比分析显示,转基因植株的根冠比显著高于对照植株,OE2的根冠比是WT的1.53倍。盐胁迫和低温胁迫也取得了类似的结果。以上结果表明转芒果MiNAC1基因可以通过增加转基因植株的根长和侧根数量提高其对干旱胁迫、盐胁迫和低温胁迫的抗性。本研究初步揭示了MiNAC1基因的功能,为深入研究MiNAC1基因参与调控芒果逆境胁迫调控网络奠定基础。NAC transcription factors play important roles in the response to abiotic stress in plants.Studying the function of the mango Mi NAC1 gene may provide genetic resources for mango stress tolerance breeding.Abiotic stresses such as drought,salt and low temperature affect the growth and development of mango.In a previous study,a Mi NAC1 gene was obtained from the transcriptome of mango stress samples,and the expression pattern analysis showed that it was related to the stress response of mango.In this study,the function of the mango Mi NAC1 gene was verified.The pBI121-MiNAC1 overexpression vector was constructed and transferred into the model plant Arabidopsis thaliana.The T3 homozygous lines were used for phenotypic observation and stress treatment.The results showed that the phenotypes of the transgenic mango MiNAC1 gene and WT wild-type Arabidopsis thaliana were similar with each other.The transgenic mango MiNAC1 gene did not affect the number of rosette leaves,bolting time,flowering time and plant height at flowering.Transgenic and WT Arabidopsis thaliana were treated with Mannitol(0,200,300,400 mmol/L),salt(0,100,150,200mmol/L) and low temperature stress at 4℃,respectively.Stress treatment was performed by comparing the changes in root length,lateral root number and root crown ratio with the transgenic and control plants.Under drought stress treatment,the root length of WT and transgenic plants was obviously inhibited with the concentration increase of mannitol treatment,but the degree of inhibition was significantly lower in transgenic lines than that of WT plants.The lateral root number increased,but the transgenic plants were significantly higher than that of WT plants.In addition,the root crown ratio of transgenic plants was also significantly higher than that of control plants.For example,under 300 mmol/L mannitol treatments,the root length increased significantly,with OE9 was 1.51 times longer than that of WT.The number of lateral roots increased significantly,with OE7 having 5 times more lateral roots

关 键 词：芒果 转录因子 MiNAC1 非生物胁迫 功能分析 

分 类 号：S667.7[农业科学—果树学]