CRISPR/Cas基因编辑技术及其在花生育种中的应用前景  

CRISPR Gene Editing Technology and Its Application Prospects in Peanut Breeding

作　　者：张金霞 刘骏 王士苗 董彦琪[1] 张栩[1] 彭东[1] Zhang Jinxia;Liu Jun;Wang Shimiao;Dong Yanqi;Zhangxu;Peng Dong(Xinxiang Academy of Agricultural Sciences,Xinxiang,453002;Jiaozuo Academy of Agriculture and Forestry Science,Jjaozuo,454150)

机构地区：[1]河南省新乡市农业科学院,新乡453002 [2]焦作市农林科学研究院,焦作454150

出　　处：《分子植物育种》2022年第15期5011-5020,共10页Molecular Plant Breeding

基　　金：河南省科技攻关计划项目(172102110147,182102110100);河南省科普行动计划项目(HNKJZK-2020-64C)共同资助。

摘　　要：随着生物技术和分子生物学的发展,基因编辑技术从锌指核酸酶(ZFN)、转录激活因子样效应因子核酸酶(TALEN)发展到应用更加简便有效的规律重复短回文序列簇(CRISPR)技术,目前正逐步应用于不同的作物育种中。本研究介绍了CRISPR基因编辑技术的基本结构和原理及其在油料作物上的初步应用,同时针对目前优质花生育种的需求,展望CRISPR技术在花生中的应用前景。With the development of biotechnology and molecular biology,gene editing technology has developed from zinc finger nuclease(ZFN)and transcription activator-like effector nuclease(TALEN)to apply of more simple and effective regular repeated short palindrome clusters(CRISPR)technology,which is currently being applied to different crop breeding.This study introduces the basic structure and principle of CRISPR gene editing technology and its preliminary application in oil crops.At the same time,in response to the current demand for high-quality peanut breeding,we look forward to the application prospects of CRISPR technology in peanuts.

关 键 词：基因编辑 CRISPR 花生 高油酸 FAD2 

分 类 号：S565.2[农业科学—作物学]