改良化学沉淀法和过氧化物酶法检测冠心病患者血清小而密低密度脂蛋白的方法学比较  

Methodological comparison of modified chemical precipitation method and peroxidase method for the testing of small dense low-density lipoprotein in serum from patients with coronary artery disease

作　　者：解晨曦[1] 毕波[1] XIE Chenxi;BI Bo(Department of Clinical Laboratory,the Fifth Affiliated Hospital of Xinjiang Medical University,Xinjiang,Urumqi 830011,China)

机构地区：[1]新疆医科大学第五附属医院检验科,新疆乌鲁木齐830011

出　　处：《中国医药科学》2022年第15期135-137,177,共4页China Medicine And Pharmacy

基　　金：省部共建中亚高发病成因与防治国家重点实验室开放课题项目(SKL-HIDCA-2020-WF4)。

摘　　要：目的 探讨改良化学沉淀法和过氧化物酶法检测冠心病患者血清小而密低密度脂蛋白(sdLDL)的方法学比较。方法 选取新疆医科大学第五附属医院2021年1—8月收治的冠心病患者82例,分别实施肝素镁沉淀法和过氧化物酶法,并比较肝素镁沉淀法和过氧化物酶法检测sdLDL-C的精密度、线性分析与sdLDL-C水平。结果 过氧化物酶法和肝素镁沉淀法检测sdLDL-C,批间、批内、总变异系数均<2.972%,精密度和线性良好。肝素镁沉淀法与过氧化物酶法均有良好的相关性,且呈正直线关系(P <0.05)。肝素镁沉淀法和过氧化物酶法测定sd LDL-C水平结果比较,差异无统计学意义(P> 0.05)。结论 肝素镁沉淀法和过氧化物酶法的相关性良好,对于冠心病患者血清sdLDL-C均有较好的检测效果,但过氧化物酶法更为快速、简便,有助于sdLDL检测标准化的实施。Objective To compare the methodology of modified chemical precipitation method and peroxidase method for the testing of small dense low-density lipoprotein(sdLDL) in serum from patients with coronary artery disease. Methods A total of 82 patients with coronary heart disease admitted to the Fifth Affiliated Hospital of Xinjiang Medical University from January to August 2021 were selected to receive tests by heparinmagnesium precipitation method and peroxidase method respectively. The precision, linearity and sdLDLcholesterol(sdLDL-C) level of sdLDL-C testing by heparin-magnesium precipitation method and peroxidase method were compared. Results The inter-batch and intra-batch total coefficients of variation were < 2.972%with good precision and linearity for sdLDL testing by the peroxidase method and the heparin-magnesium precipitation method. Both the heparin-magnesium precipitation method and the peroxidase method showed good correlation and positive linear relationship(P < 0.05). There was no statistically significant difference between the sdLDL-C levels measured by the heparin-magnesium precipitation method and the peroxidase method(P > 0.05). Conclusion Heparin-magnesium precipitation method and peroxidase method have good correlation and both can well test serum sdLDL-C in patients with coronary artery disease, but peroxidase method is more rapid and simple in operation, which is conducive to the implementation of standardization of sdLDL testing.

关 键 词：肝素镁沉淀法 过氧化物酶法 冠心病 低密度脂蛋白 

分 类 号：R446.1[医药卫生—诊断学] R541.4[医药卫生—临床医学]