花旗松素对高糖诱导的人肾小管上皮细胞炎症损伤的影响及其机制  

Influence of taxifolin on high glucose-induced inflammatory injury of human renal tubular epithelial cells and its mechanism

作　　者：陈静[1] 张进[2] CHEN Jing;ZHANG Jin(Academic Affairs Section,Baoji Vocational&Technical College,Baoji 721003,Shaanxi,China;Department of Endocrinology,Baoji People′s Hospital,Baoji 721000,Shaanxi,China)

机构地区：[1]宝鸡职业技术学院教务处,陕西省宝鸡市721003 [2]宝鸡市人民医院内分泌科,陕西省宝鸡市721000

出　　处：《广西医学》2022年第14期1629-1635,共7页Guangxi Medical Journal

基　　金：陕西省宝鸡市哲学社会科学项目(BJSKZX-202113)。

摘　　要：目的 探讨花旗松素对高糖诱导的人肾小管上皮细胞炎症损伤的影响,并分析其可能机制。方法 将肾小管上皮细胞分为Con组(5.5 mmol/L葡萄糖处理细胞)、HG组(25 mmol/L葡萄糖处理细胞)、HG+花旗松素-L组(25 mmol/L葡萄糖+1μmol/L花旗松素处理细胞)、HG+花旗松素-M组(25 mmol/L葡萄糖+10μmol/L花旗松素处理)、HG+花旗松素-H组(25 mmol/L葡萄糖+100μmol/L花旗松素处理)、HG+pcDNA组(25 mmol/L葡萄糖处理转染pcDNA的细胞)、HG+pcDNA-circ_0001445组(25 mmol/L葡萄糖处理转染pcDNA-circ_0001445的细胞)、HG+花旗松素+si-NC组(25 mmol/L葡萄糖+100μmol/L花旗松素处理转染si-NC的细胞)、HG+花旗松素+si-circ_0001445组(25 mmol/L葡萄糖+100μmol/L花旗松素处理转染si-circ_0001445的细胞)。采用流式细胞术分析各组肾小管上皮细胞的凋亡率;ELISA检测各组细胞上清液中白细胞介素6(IL-6)和肿瘤坏死因子α(TNF-α)水平;Western blot检测各组肾小管上皮细胞的活化型Caspase(cleaved Caspase)-3、cleaved Caspase-9蛋白表达量;实时荧光定量PCR检测各组肾小管上皮细胞的circ_0001445表达量。结果 与Con组比较,HG组的细胞凋亡率、IL-6水平、TNF-α水平、cleaved Caspase-3和cleaved Caspase-9蛋白表达量均增加,circ_0001445表达量降低(均P<0.05);与HG组比较,HG+花旗松素-L组、HG+花旗松素-M组、HG+花旗松素-H组的细胞凋亡率、IL-6水平、TNF-α水平、cleaved Caspase-3和cleaved Caspase-9蛋白表达量均降低,circ_0001445表达量均升高(均P<0.05),且花旗松素剂量越高其作用越明显。与HG+pcDNA组比较,HG+pcDNA-circ_0001445组的细胞凋亡率、IL-6水平、TNF-α水平、cleaved Caspase-3和cleaved Caspase-9蛋白表达量均降低(均P<0.05);与HG+花旗松素+si-NC组比较,HG+花旗松素+si-circ_0001445组的细胞凋亡率、IL-6水平、TNF-α水平、cleaved Caspase-3和cleaved Caspase-9蛋白表达量均升高(均P<0.05)。结论 花旗松素可呈剂量依赖性地减轻高糖Objective To investigate the influence of taxifolin on high glucose-induced inflammatory injury of human renal tubular epithelial cells, and to analyze its possible mechanism.Methods Renal tubular epithelial cells were assigned to Con group(cells processed with 5.5 mmol/L glucose), HG group(cells processed with 25 mmol/L glucose), HG + taxifolin-L group(cells processed with 25 mmol/L glucose+1 μmol/L taxifolin), HG+taxifolin-M group(cells processed with 25 mmol/L glucose+10 μmol/L taxifolin), HG+taxifolin-H group(cells processed with 25 mmol/L glucose+100 μmol/L taxifolin), HG+pcDNA group(pcDNA-transfected cells processed with 25 mmol/L glucose), HG+pcDNA-circ_0001445 group(pcDNA-circ_0001445-transfected cells processed with 25 mmol/L glucose), HG+taxifolin+si-NC group(si-NC-transfected cells processed with 25 mmol/L glucose+100 μmol/L taxifolin), or HG+ taxifolin+si-circ_0001445 group(si-circ_0001445-transfected cells processed with 25 mmol/L glucose+100 μmol/L taxifolin). The flow cytometry was employed to analyze the apoptosis rate of renal tubular epithelial cells. The ELISA was used to detect the levels of interleukin 6(IL-6), and tumor necrosis factor α(TNF-α) in cell supernate of various groups. The Western blot was used to detect protein expressions of cleaved Caspase-3, cleaved Caspase-9 of renal tubular epithelial cells in various groups. The real-time fluorescent quantitative PCR was employed to detect expression of circ_0001445 of renal tubular epithelial cells in various groups.Results Compared with the Con group, the cell apoptosis rate, IL-6 level, TNF-α level, the protein expressions of cleaved Caspase-3 and cleaved Caspase-9 in the HG group were increased, whereas the expression of circ_0001445 in the HG group was decreased(all P<0.05). Compared to the HG group, the cell apoptotic rate, IL-6 level, TNF-α level, and the protein expressions of cleaved Caspase-3 and cleaved Caspase-9 in the HG+taxifolin-L, HG+taxifolin-M and HG+taxifolin-H groups were decreased, whereas the circ_0001445 exp

关 键 词：花旗松素 肾小管上皮细胞 炎症损伤 高糖 细胞凋亡 炎性因子 环状RNA circ_0001445 

分 类 号：R284[医药卫生—中药学] R692[医药卫生—中医学]