microRNA-96与脓毒症新生儿炎症反应的关系研究  

Relationship between microRNA-96 and inflammatory response in neonatal sepsis

作　　者：张春磊[1] 李秀婷[1] 刘娜[1] 张成元[1] ZHANG Chunlei;LI Xiuting;LI Na;ZHANG Chengyuan(Department of Pediatrics,Weifang Maternal and Child Health Hospital,Weifang,Shandong 261011,China)

机构地区：[1]潍坊市妇幼保健院儿科,山东潍坊261011

出　　处：《国际检验医学杂志》2022年第17期2131-2134,2140,共5页International Journal of Laboratory Medicine

基　　金：山东省潍坊市卫生健康委员会科研项目(WFWSJK-2020-136)。

摘　　要：目的分析微小RNA-96(miR-96)与新生儿脓毒症(NS)炎症反应的关系。方法将2018年6月至2020年4月在该院治疗的65例NS患儿作为NS组,将同期65例非感染性全身炎症反应综合征(SIRS)患儿作为非感染性SIRS组,另选50例健康体检儿童作为健康组。采用实时定量聚合酶链式反应(RT-PCR)法测定miR-96水平,使用酶联免疫吸附试验(ELISA)测定血清炎性因子[白细胞介素-1β(IL-1β)、IL-6、肿瘤坏死因子-α(TNF-α)]水平,采用Pearson相关分析miR-96与炎症反应指标的相关性;随访后,将NS组分为存活组、死亡组,对比不同预后患儿血清各指标差异,绘制受试者工作特征(ROC)曲线,分析miR-96对NS患儿预后的预测价值。结果NS组序贯器官衰竭评估评分、急性生理学和慢性健康评价Ⅱ(APACHEⅡ)评分比非感染性SIRS组高,差异均有统计学意义(P<0.05);NS组miR-96水平比非感染性SIRS组、健康组低,IL-1β、IL-6、TNF-α水平比非感染性SIRS组、健康组高,差异均有统计学意义(P<0.05);Pearson相关分析结果显示,miR-96水平与IL-1β、IL-6、TNF-α均呈负相关(r<0,P<0.05);死亡组miR-96水平比存活组低,IL-1β、IL-6、TNF-α水平比存活组高,差异均有统计学意义(P<0.05);ROC曲线结果显示,miR-96预测NS患儿病死的AUC为0.903(95%CI:0.846~0.978),以0.75为临界值,其灵敏度、特异度分别为0.894、0.855,约登指数为0.749。结论NS患儿miR-96水平下调,炎性因子水平上调,二者呈负相关;且miR-96水平对NS预后有一定的预测价值。Objective To analyze the relationship between microRNA-96(miR-96)and inflammatory response in neonatal sepsis(NS).Methods A total of 65 NS children from the hospital from June 2018 to April 2020 were taken as the NS group,65 children with non-infectious systemic inflammatory response syndrome(SIRS)in the same period were included as the non-infectious SIRS group,and another 50 healthy children who underwent the health examination were selected as the health group.The level of serum miR-96 was determined by real-time quantitative PCR(RT-PCR),and the levels of serum inflammatory factors[interleukin-1β(IL-1β),interleukin-6(IL-6),tumor necrosis factor(TNF-α)]were determined by enzyme linked immunosorbent assay.Pearson correlation was used to analyze the correlation between serum miR-96 and inflammatory reaction indexes.The NS group was divided into survival group and death group after the follow-up,and the differences of serum indexed in children with different prognosis were compared.The receiver operating characteristic(ROC)curve was drawn,and the prognostic value of miR-96 in children with NS was analyzed.Results The sequential organ failure assessment(SOFA)score and acute physiology and chronic health evaluation(APACHEⅡ)score in the NS group were higher than those in the non-infectious SIRS group,and the differences were statistically significant(P<0.05).The level of miR-96 in the NS group was lower than those in the non-infectious SIRS group and the health group,the levels of IL-1β,IL-6,TNF-αwere higher than those in the non-infectious SIRS group and health group,and the differences were statistically significant(P<0.05).Pearson correlation analysis results showed that miR-96 level was negatively correlated with IL-1β,IL-6,and TNF-αlevels(r<0,P<0.05).The level of miR-96 in the death group was lower than that in the survival group,and the levels of IL-1β,IL-6 and TNF-αwere higher than those in the survival group,and the differences were statistically significant(P<0.05).The ROC curve results showed tha

关 键 词：微小RNA-96 新生儿脓毒症 炎症反应 预后 

分 类 号：R446.61[医药卫生—诊断学]