基于ITS序列对几株野生大型真菌的鉴定  

Identification of Several Wild Fungi Based on ITS Sequence

作　　者：朱链 侯怡铃[1,2] 白鑫 丁祥[2,3] ZHU Lian;HOU Yi-ling;BAI Xin;DING Xiang(College of Life Sciences,China West Normal University,Nanchong Sichuan 637009,China;Key Laboratory of Southwest China Wildlife Resources Conservation of the Ministry of Education,China West Normal University,Nanchong Sichuan 637009,China;College of Environmental Science and Engineering,China West Normal University,Nanchong Sichuan 637009,China)

机构地区：[1]西华师范大学生命科学学院,四川南充637009 [2]西华师范大学西南野生动植物资源保护教育部重点实验室,四川南充637009 [3]西华师范大学环境科学与工程学院,四川南充637009

出　　处：《西华师范大学学报（自然科学版）》2022年第3期253-257,共5页Journal of China West Normal University(Natural Sciences)

基　　金：四川省科技厅重点研发项目(2018NZ0055)。

摘　　要：采用传统分类法与ITS序列分析法对采自四川老君山地区的6株野生真菌进行分类鉴定。依据形态特征初步鉴定这6株菌株有1份棒束孢属(Isaria)(1号),1份湿伞菌属(Hygrocybe)(3号),1份珊瑚菌属(Clavaria)(4号),1份银耳菌属(Pseudohydnum)(5号),1份小菇菌属(Mycena)(8号),1份拟锁瑚菌属(Clavulinopsis)(26号)。为了提高鉴定的准确性,对6株菌株总DNA的PCR扩增产物测序,然后以N-J法构建系统进化树,以探索其与已知菌种的相关性。结果表明:1号(OL617416)真菌样品为大草蝉草菌(Isaria cicadae),3号(OL617418)真菌样品为鸡油菌状湿伞(Hygrocybe Cantharellus),4号(OL617419)真菌样品为碎白珊瑚菌(Clavaria fragilis),5号(OL617420)真菌样品为胶质刺银耳(Pseudohydnum gelatinosum),8号(OL616140)真菌样品为盔盖小菇菌(Mycena galericulate),26号(OL616134)真菌样品为环沟拟锁瑚菌(Clavulinopsis sulcate)。Traditional classification method and ITS sequence analysis method are adopted to classify and identify the six wild fungi collected in Laojun Mountain of Sichuan.According to their morphological characteristics,it is preliminarily determined that they are Cordycipitaceae Isaria(For 1 copy,No.1),Hygrocybe(For 1 copy,No.3)and Clavaria(For 1 copy,No.4),Pseudohydnum(For 1 copy,No.5),Mycena(For 1 copy,No.8)and Clavulinopsis(For 1 copy,No.26).PCR amplification product of the total DNA is sequenced to improve the accuracy of identification and a phylogenetic tree is constructed by N-J method to explore its correlation with known strains.The final result indicate that No.1(OL617416)is Cordyceps cicadae;No.3(OL617418)is Hygrocybe Cantharellus;No.4(OL617419)is Clavaria fragilis;No.5(OL617420)is Pseudohydnum gelatinosum;No.8(OL616140)is Mycena galericulate and No.26(OL616134)is Clavulinopsis sulcate.

关 键 词：真菌 分类鉴定 RDNA ITS 测序 老君山 

分 类 号：Q949.329[生物学—植物学]