基于抑菌实验优化清消五味汤提取工艺  

Optimization of extraction process of Qingxiao Wuwei Decoction based on antibacterial experiment

作　　者：陈新荣[1] 张谦[2] Chen Xinrong;Zhang Qian(Department of Clinical Laboratory,Bayannaoer Hospital,Bayannaoer 015000,Inner Mongolia Autonomous Region,China;The Center for New Drug Safety Evaluation and Research,School of Pharmacy,Inner Mongolia Medical University,Hohhot 010110,Inner Mongolia Autonomous Region,China)

机构地区：[1]巴彦淖尔市医院检验科,内蒙古自治区巴彦淖尔015000 [2]内蒙古医科大学新药安全评价研究中心,药学院,内蒙古自治区呼和浩特010110

出　　处：《实用检验医师杂志》2022年第2期164-167,共4页Chinese Journal of Clinical Pathologist

基　　金：内蒙古自治区自然科学基金(2021MS08087);内蒙古自治区高等学校科学研究项目(NJZY21632)。

摘　　要：目的基于抑菌实验进行清消五味汤的醇提取工艺优化。方法采用琼脂打孔法抑菌实验,以革兰阴性(G^(-))菌(大肠杆菌)和革兰阳性(G^(+))菌(金黄色葡萄球菌)的抑菌圈直径综合评分作为考察指标,采用L9(34)正交试验设计,以料液比A(分别为1︰6、1︰8、1︰10kg/L)、提取次数B(分别为1、2、3次)、提取时间C(分别为0.5、1、1.5h)、乙醇浓度D(分别为45%、60%、75%)为考察因素,进行清消五味汤醇提取工艺的优化。结果9种清消五味汤提取物(a~i)对大肠杆菌均无明显抑制作用,对金黄色葡萄球菌的抑菌直径分别为提取物a(10.89±0.23)cm,提取物b(14.25±0.55)cm,提取物c(16.69±0.79)cm,提取物d(13.17±0.34)cm,提取物e(14.93±0.37)cm,提取物f(15.39±0.88)cm,提取物g(14.91±0.26)cm,提取物h(14.26±0.54)cm和提取物i(16.99±0.71)cm。采用L9(34)正交试验确定清消五味汤最优提取工艺为A3B3C3D3,即料液比为1︰10 kg/L,提取次数为3次,提取时间为1.5h,乙醇浓度为75%。结论实验优化所得的清消五味汤醇提取工艺稳定、可行,能为清消五味汤的进一步临床应用提供科学依据。Objective To optimize the alcohol extraction process of Qingxiao Wuwei Decoction based on antibacterial experiment.Methods The antibacterial experiment by agar punching method and the comprehensive score of the inhibition zone diameter of Gram-negative(G^(-))bacteria(Escherichia coli)and Gram-positive(G^(+))bacteria(Staphylococcus aureus)were used as the investigation indexes.The L9(34)orthogonal experimental design was adopted and the ratio of solid to liquid A(1︰6,1︰8 and 1︰10 kg/L,respectively),extraction times B(1,2 and 3 times,respectively),extraction time C(0.5,1 and 1.5 h,respectively)and ethanol concentration D(45%,60% and 75%,respectively)were selected as the investigated factors to optimize the ethanol extraction process of Qingxiao Wuwei Decoction.Results Nine kinds of Qingxiao Wuwei Decoction extracts(a-i)had no significant inhibitory effect on Escherichia coli,and the antibacterial diameters against Staphylococcus aureus were as follows:extract a(10.89±0.23)cm,extract b(14.25±0.55)cm,extract c(16.69±0.79)cm,extract d(13.17±0.34)cm,extract e(14.93±0.37)cm,extract f(15.39±0.88)cm,extract g(14.91±0.26)cm,extract h(14.26±0.54)cm and extract i(16.99±0.71)cm,respectively.At the same time,the L9(34)orthogonal test was used to determine that the optimal extraction process of Qingxiao Wuwei Decoction was A3B3C3D3(the solid-liquid ratio was 1︰10 kg/L,the extraction times were 3 times,the extraction time was 1.5 h,and the ethanol concentration was 75%).Conclusion The optimized alcohol extraction process of Qingxiao Wuwei Decoction is stable and feasible,which can provide scientific basis for the further clinical application of Qingxiao Wuwei Decoction.

关 键 词：清消五味汤 抑菌试验 综合评分 工艺优化 

分 类 号：R283.6[医药卫生—中药学]