基于UPLC-Q-Exactive Orbitrap-MS的栀子柏皮汤化学成分分析  

Analysis of Chemical Components of Zhi-Zi-Bo-Pi Decoction Based on UPLC-Q-Exactive Orbitrap-MS

作　　者：景涵 冯芳[1,2] JING Han;FENG Fang(Department of Pharmaceutical Analysis,China Pharmaceutical University,Jiangsu Nanjing 210009;Key Laboratory of Drug Quality Control and Pharmacovigilance(Ministry of Education),Jiangsu Nanjing 210009,China)

机构地区：[1]中国药科大学药物分析教研室,江苏南京210009 [2]药物质量与安全预警教育部重点实验室,江苏南京210009

出　　处：《广州化工》2022年第16期81-87,共7页GuangZhou Chemical Industry

基　　金：国家自然科学基金资助项目(No:81872993)。

摘　　要：运用超高效液相色谱-四级杆-静电场轨道阱质谱(UPLC-Q-Exactive Orbitrap-MS)技术对栀子柏皮汤化学成分进行了快速、全面分析。采用XBridge BEH C色谱柱(2.1 mm×100 mm,2.5μm),以0.1%甲酸水-乙腈为流动相进行梯度洗脱,流速0.3 mL/min,进样量3μL;运用加热电喷雾电离源(HESI)在正、负离子模式下采集数据。通过比对质谱数据和对照品保留时间等,共鉴定出栀子柏皮汤中的146个化学成分,为后续的质量控制和作用机制研究奠定了基础。A fast and comprehensive analysis of chemical components in Zhi-Zi-Bo-Pi decoction was performed by UPLC-Q-Exactive Orbitrap-MS.The analysis was performed on XBridge BEH Ccolumn(2.1 mm×100 mm,2.5μm)with the mobile phase consisting of 0.1%formic acid water solution-acetonitrile for gradient elution at a flow rate of 0.3 mL/min and injection volume of 3μL.MS data in positive and negative mode was obtained by heating-electrospray ion source.By comparing the MS data in databases and the retention time of reference compounds,a total of 146 components were identified,which provides a support for the following quality control and mechanism study.

关 键 词：栀子柏皮汤 成分分析 超高效液相色谱-四级杆-静电场轨道阱质谱 

分 类 号：R917[医药卫生—药物分析学]