生脉饮对阿霉素损伤心肌细胞的保护作用研究  

Research of Protective Effect of Shengmaiyin on Cardiomyocyte Injury Induced by Adriamycin

作　　者：崔海峰 彭博[3] 冯淑怡[1,2] 黄颖 石晓路[1,2] 武乾 孙明杰[1,2] 孙丽华 CUI Haifeng;PENG Bo;FENG Shuyi;HUANG Ying;SHI Xiaolu;WU Qian;SUN Mingjie;SUN Lihua(Beijing Key Laboratory of TCM Basic Research on Prevention and Treatment of Major Disease,Beijing 100700,China;Experimental Research Center,China Academy of Chinese Medical Sciences,Beijing 100700,China;Institute of Chinese Materia Medica,China Academy of Chinese Medical Sciences,Beijing 100700,China)

机构地区：[1]北京市中医药防治重大疾病基础研究重点实验室,北京100700 [2]中国中医科学院医学实验中心,北京100700 [3]中国中医科学院中药研究所,北京100700

出　　处：《中国中医基础医学杂志》2022年第8期1245-1248,1273,共5页JOURNAL OF BASIC CHINESE MEDICINE

基　　金：国家自然科学基金青年基金项目(82004244)-基于CaMKⅡ信号通路调控探讨生脉散干预糖尿病小鼠心肌钙稳态及电生理重构机制;国家自然科学基金青年基金项目(81803983)-基于AMPK激活的线粒体自噬通路探讨生脉饮抗心力衰竭的作用机制;中央级公益性科研院所基本科研业务费专项资金资助项目(ZZ2018002)-生脉饮增强阿霉素抗乳腺癌作用减轻其心肌毒性的实验研究;国家重点研发计划(2018YFC1708105)-苗药大品种开喉剑喷雾剂、金骨莲胶囊的关键技术提升与应用示范;国家重点研发计划(2018YFC1708100)-开喉剑与金骨莲的临床前有效性和安全性研究。

摘　　要：目的:观察不同浓度生脉饮对阿霉素(adriamycin,ADM)损伤大鼠心肌细胞的干预作用并初步探讨其可能机制。方法:以H9c2大鼠心肌细胞为研究对象,应用细胞计数试剂(cell counting kit-8,CCK-8)检测不同浓度生脉饮对H9c2细胞的毒性作用,筛选ADM损伤H9c2细胞最适浓度确定损伤模型,设正常对照组、ADM组、ADM+不同浓度生脉饮组,CCK-8检测生脉饮对损伤细胞的保护作用,应用WST-1法检测各组超氧化物歧化酶(superoxide dismutase,SOD)的变化,应用TBA法检测各组丙二醛(malondialdehyde,MDA)的变化。结果:生脉饮(25、200、1600μg/mL)作用H9c2细胞72 h能够促进细胞增殖(P<0.05),1.5625~100μmol/L ADM作用H9c2细胞均呈现明显的毒性并存在时间依赖关系(P<0.01),生脉饮400μg/mL对ADM(1μmol/L)损伤24 h H9c2细胞有保护作用(P<0.05),ADM(1μmol/L)损伤48 h、生脉饮100、200μg/mL组细胞内SOD活力高于ADM组(P<0.05),ADM(1μmol/L)损伤72 h、生脉饮50、200μg/mL组细胞内SOD活力增加(P<0.05,P<0.01),不同时间点生脉饮各剂量组MDA含量均较ADM组降低(P<0.01)。结论:生脉饮对ADM损伤H9c2细胞具有一定的保护作用,可能与其增加SOD活力、降低MDA含量相关。Objective:To observe the interventional effects of Shengmai decoction on the proliferation of cardiomyocyte injured by Adriamycin(ADM),and to explore the possible mechanisms.Methods:CCK-8 was used to observe the cytotoxicity of Shengmai decoction on H9c2 rat cardiomyocyte.The optimal concentration of ADM damage to H9c2 cells was screened.The experimental groups were control group,ADM group and ADM+different concentration of Shengmaiyin groups.CCK-8 analysis was employed to evaluate the proliferation effect of cells after the injury.After the damage,SOD and MDA contents were respectively detected by WST-1 and TBA methods.Results:Shengmaiyin(25,200,1600μg/mL)enhanced the proliferation of H9c2 cells after 72h cell culture(P<0.05).ADM(1.5625~100μmol/L)presented significant toxicity on H9c2 cell(P<0.05),and the toxicity level depended on the duration of ADM cultured with cells.Shengmaiyin(400μg/mL)appeared protective effect against the toxicity of ADM(P<0.05).Compared with ADM group,SOD activity of 100,200μg/mL Shengmaiyin groups were increased after 48h ADM cultured with cells(P<0.05).SOD activity of 50,200μg/mL Shengmaiyin groups were also increased after 72h ADM cultured with cells.Compared with ADM group,MDA content of all dose of Shengmaiyin groups were decreased(P<0.01).Conclusion:Shengmaiyin could protect the cardiomyocyte against the injury of ADM.The protective effect might relate with the increasing of SOD activity and the decreasing of MDA content.

关 键 词：生脉饮 阿霉素 心肌细胞损伤 细胞存活率 

分 类 号：R289.5[医药卫生—方剂学]