高温胁迫下芽孢杆菌对条斑紫菜生长及生理的影响  

Effects of Bacillus sp.on Growth and Physiology of Laver Pyropia yezoensis Under High Temperature Stress

作　　者：欧阳乐飞 王林华 邓高威 张庆起 高焕 阎斌伦 OUYANG Lefei;WANG Linhua;DENG Gaowei;ZHANG Qinghe;GAO Huan;YAN Binlun(Jiangsu Key Laboratory of Marine Bioresources and Environment/Jiangsu Key Laboratory of Marine Biotechnology School of Marine Science and Fisheries,Jiangsu Ocean University,Lianyungang 222005,China;Co-Innovation Center of Jiangsu Marine Bio-industry Technology,Jiangsu Ocean University,Lianyungang 222005,China;Ganyu-Jiaxin Aquculture Limited Company of Lianyungang,Lianyungang 222100,China;The Jiangsu Provincial Platform for Conservation and Utilization of Agricultural Germplasm,Nanjing 210014,China)

机构地区：[1]江苏海洋大学,江苏省海洋生物技术重点实验室,江苏省海洋生物资源与生态环境重点实验室,江苏连云港222005 [2]江苏省海洋生物产业技术协同创新中心,江苏连云港222005 [3]连云港赣榆佳信水产开发有限公司,江苏连云港222100 [4]江苏省农业种质资源保护与利用平台,江苏南京210014

出　　处：《水产学杂志》2022年第4期81-86,共6页Chinese Journal of Fisheries

基　　金：国家现代农业产业体系-藻类体系(CARS-50);江苏省水产三新工程(Y2017-32);江苏海洋大学研究生创新项目(SJCX19-0995);江苏省优势学科建设工程资助项目.

摘　　要：将300 mL紫菜振荡液涂布于Zobell 2216E海水培培养基中接种0.2 g无菌处理过的条斑紫菜(Pyropia yezoensis)叶状体与约3×106 cells/mL Bacillus sp.LPyS1,在22℃、盐度30、光强3500 lx,光周期12L∶12D,充气条件下培养0 h、6 h、12 h、24 h、48 h和72 h取样,测定其相对生长率(RGR)、可溶性蛋白、藻胆蛋白、游离脯氨酸(Pro)和丙二醛(MDA)含量,以及超氧化物岐化酶(SOD)和过氧化物酶(POD)活性。以仅接种条斑紫菜叶状体、相同培养作为对照,探究高温胁迫下藻际附生菌对条斑紫菜叶状体生长和成活的影响。结果发现,与对照组相比,24 h时藻菌共培养组条斑紫菜的RGR、MDA和藻胆蛋白水平及SOD、POD活性均显著增加(P<0.05),但渗透调节物质Pro与可溶性蛋白含量均显著低于对照组(P<0.05);而72 h共培养组的RGR无显著变化(P>0.05),POD、MDA、藻胆蛋白和Pro均显著高于对照组(P<0.05);连续培养4 d后两组条斑紫菜均出现腐烂现象,对照组藻体腐烂更为严重。研究表明:高温条件下短期内藻际芽孢杆菌可以增强条斑紫菜的抗逆性和生长,而后培养时间的延长会加剧渗透压和活性氧胁迫,芽孢杆菌对条斑紫菜的促生长作用随其抗逆能力的减弱而缓慢下降,说明该菌株的生态功能因环境变化而发生了改变。Relative growth rate(RGR),soluble protein,contents of phycobiliprotein,free proline(Pro),malondialdehyde(MDA),activities of superoxide dismutase(SOD)and peroxidase(POD)were determined in laver Pyropia yezoensis fronds cultured in Zobell 2216E seawater culture medium and inoculated 0.2 g of sterile laver and about 3×106 cells/mL Bacillus sp.LPySl at 22℃,salinity of 30,light intensity of 3,500 lx,photoperiod of 12L∶12D,under aerated conditions for 0 h,6 h,12 h,24 h,48 h,and 72 h,with only inoculation of laver fronds as a control,to evaluate the effect of epiphytic bacteria on algae under high temperature stress.The results showed that there were significantly higher RGR,contents of MDA,phycobiliprotein,Pro,activities of SOD and POD in the 24 h treat group than those in the control group(P<0.05),without significant difference between the 72 h treat group and the control group(P>0.05).Four days culture later,the laver was rotted in the two groups,more severely in the control group,indicating that Bacillus sp.LPyS1 enhanced the stress resistance and promoted the growth of laver in a short period of time at high temperature.Then the elapse of the culture time intensified the osmotic pressure and reactive oxygen stress,and the growth-promoting effect of Bacillus on laver was slowly decreased as its resistance to stress weakens.The finding shows that the ecological function of the laver has changed with environmental changes.

关 键 词：条斑紫菜 芽孢杆菌 高温胁迫 生长 生理 

分 类 号：S968.4[农业科学—水产养殖]