分子印迹技术用于抗生素残留检测的研究进展  

Research Advances of Molecular Imprinting Technology for the Detection of Antibiotics Residues

作　　者：赵广丽 张悦 孙大妮 周娜[2] 韩铠营 闫李霞 于佳洛 宋志花 李桂生[1] ZHAO Guang-li;ZHANG Yue;SUN Da-ni;ZHOU Na;HAN Kai-ying;YAN Li-xia;YU Jia-luo;SONG Zhi-hua;LI Gui-sheng(Shandong Provincial High-efficiency Collaborative Innovation Center for Novel Formulations and Biotechnology Drug Research,Key Laboratory of Molecular Pharmacology and Drug Evaluation,Ministry of Education,School of Pharmacy,Yantai University,Yantai 264005,China;CAS Key Laboratory of Coastal Environmental Processes and Ecological Remediation,Yantai Institute of Coastal Zone Research,Chinese Academy of Sciences,Yantai 264003,China;Yantai No.2 Middle School of Shandong Province,Yantai 264000,China)

机构地区：[1]烟台大学药学院,分子药理和药物评价教育部重点实验室,新型制剂与生物技术药物研究山东省高效协同创新中心,山东烟台264005 [2]中国科学院烟台海岸带研究所,中国科学院海岸带环境过程与生态修复重点实验室,山东烟台264003 [3]山东省烟台第二中学,山东烟台264000

出　　处：《化学试剂》2022年第9期1350-1359,共10页Chemical Reagents

基　　金：山东省自然科学基金项目(ZR2020QB105);中国科学院仪器设备功能开发技术创新项目;烟台市科技创新发展计划项目(2020MSGY112)。

摘　　要：模拟酶-底物或抗原-抗体特异性结合原理的分子印迹技术(MIT)在目标物识别和检测中应用广泛。MIT制备的分子印迹聚合物(MIPs)为吸附剂的分子印迹固相萃取(MISPE)和基于MIPs的传感分析均已广泛应用于抗生素的分析检测中。简要介绍了抗生素MIPs的几种新兴制备技术,包括表面印迹、纳米印迹、活性可控自由基聚合、多模板印迹、多功能单体印迹和虚拟模板印迹;然后,概述了基于MIPs的3种固相萃取(SPE)模式,即传统装柱固相萃取、磁固相萃取、分散固相萃取,以及基于MIPs传感器的基本原理。随后,重点综述了近5年MISPE和基于MIPs的电化学和荧光传感器用于环境和食品等样品中的各种抗生素残留检测的研究进展,包括磺胺类、喹诺酮类、四环素类等。最后,对抗生素MIPs的制备和应用进行了展望。Molecular imprinting technology(MIT),which mimics the specific binding principle of enzyme-substrate or antigen-antibody,is widely used in target recognition and detection.Molecularly imprinted polymers(MIPs)based sensing assays and molecularly imprinted solid-phase extraction(MISPE)fabricated from MIPs(prepared by MIT)as adsorbents,have been widely applied in the detection of antibiotics.This review briefly introduced several emerging preparation techniques for antibiotics MIPs,including surface imprinting,nanoimprinting,living-controlled radical polymerization,multitemplate imprinting,multifunctional monomer imprinting,and dummy template imprinting.Solid-phase extraction(SPE)modes,namely traditional column-packed SPE,magnetic SPE,dispersive SPE,and the basic principle of MIPs based sensors were outlined.Subsequently,the recent 5-year application advances of MISPE and MIPs-based electrochemical and fluorescence sensors in the detection of various antibiotics residues,such as sulfonamides,quinolones and tetracyclines in environmental,food and other samples were highlighted.Finally,the preparation and application of antibiotics MIPs were prospected.

关 键 词：抗生素 印迹技术 分子印迹聚合物 固相萃取 传感器 

分 类 号：R917[医药卫生—药物分析学]