巴西橡胶树胶乳中DUF1262结构域蛋白结构与基因表达分析  

Structural and Gene Expression Analysis of a DUF1262 Domain Protein in Latex from Hevea brasiliensis

作　　者：代龙军[1] 刘明洋 阳江华[1] 周凯 郭冰冰 杨洪[1] 王立丰[1,2] DAI Longjun;LIU Mingyang;YANG Jianghua;ZHOU Kai;GUO Bingbing;YANG Hong;WANG Lifeng(Key Laboratory of Biology and Genetic Resources of Rubber Tree,Ministry of Agriculture and Rural Affairs,State Key Laboratory Incubation Base for Cultivation&Physiology of Tropical Crops,Danzhou Investigation&Experiment Station of Tropical Crops,Ministry of Agriculture and Rural Affairs,Rubber Research Institute,Key Laboratory of Materials Engineering for High Performance Natural Rubber,Hainan Province,Chinese Academy of Tropical Agricultural Sciences,Haikou 571101;School of Plant Protection,Key Laboratory of Green Prevention and Control of Tropical Plant Diseases and Pests(Hainan University),Ministry of Education,Hainan University,Haikou 570228)

机构地区：[1]农业农村部橡胶树生物学与遗传资源利用重点实验室,省部共建国家重点实验室培育基地-海南省热带作物栽培生理学重点实验室,农业农村部儋州热带作物科学观测实验站,海南省高性能天然橡胶材料工程重点实验室,中国热带农业科学院橡胶研究所,海口571101 [2]海南大学,海口570228

出　　处：《植物研究》2022年第5期802-810,共9页Bulletin of Botanical Research

基　　金：国家自然科学基金面上项目(31870577)。

摘　　要：为了揭示天然橡胶生物合成酶互作蛋白结构及其在天然橡胶生物合成过程中的功能。本研究以橡胶树胶乳橡胶粒子总蛋白为研究对象,采用免疫共沉淀实验技术以天然橡胶合成关键酶顺式-异戊二烯基转移酶(CPT)抗体从胶乳中捕获了1个含DUF1262结构域的未知功能蛋白。生物信息学分析表明橡胶树基因组中包含50个编码含DUF1262结构域蛋白的基因序列;蛋白质相互作用网络分析表明DUF1262结构域蛋白可能参与调节信号转导或转录调控等过程;荧光定量PCR结果表明编码该蛋白基因的转录本在根、叶、花、枝和胶乳等组织中广泛分布,但在胶乳中表达较低,在树皮表达较高;水杨酸、脱落酸、过氧化氢及干旱处理可增强该基因在叶片中的转录水平。本研究证明DUF1262参与橡胶树逆境反应等生理过程,为揭示胶乳生物合成调控机制提供新线索。To reveal the structure and function of natural rubber biosynthase interacting proteins in natural rubber biosynthesis,the total protein of latex rubber particles of Hevea brasiliensis was used as material,and an unknown functional protein containing DUF1262 domain was captured by immunoprecipitation assay using an antibody targeting CPT,a key enzyme for natural rubber biosynthesis.Bioinformatics analysis showed that there were 50 gene sequences encoding DUF1262 domain proteins in the rubber tree genome,and protein interaction network analysis suggested that DUF1262 domain proteins might be involved in signal transduction and transcriptional regulation.Fluorescence quantitative PCR results showed that the transcription of the gene encoding this protein was widely distributed in roots,leaves,flowers,branches,latex and other tissues respectively,andthe expression was low in latex and but high in bark.Salicylic acid,abscisic acid,hydrogen peroxide and drought treatment could enhance the transcription level of this gene in leaves respectively.This study demonstrated that the DUF1262 protein was involved in stress response and other physiological processes of rubber tree,providing new clues for revealing the regulation mechanism of latex biosynthesis.

关 键 词：巴西橡胶树 DUF1262结构域 基因表达 天然橡胶合成 

分 类 号：S794.1[农业科学—林木遗传育种]