番茄褪绿病毒对MED烟粉虱成虫在不同寄主植物上的寄主选择性和取食行为的影响  

Effects of Tomato chlorosis virus on the host preference and feeding behaviors of Bemisia tabaci MED(Hemiptera:Aleyrodidae)adults on different host plants

作　　者：范晓凡 刘勇[2] 谭新球[2] 张战泓[3] 张卓[2] 史晓斌[2] 张德咏 FAN Xiao-Fan;LIU Yong;TAN Xin-Qiu;ZHANG Zhan-Hong;ZHANG Zhuo;SHI Xiao-Bin;ZHANG De-Yong(Subcollege of Longping,Graduate School of Hunan University,Changsha 410125,China;Institute of Plant Protection,Hunan Academy of Agricultural Sciences,Changsha 410125,China;Institute of Vegetable Crops,Hunan Academy of Agricultural Sciences,Changsha 410125,China)

机构地区：[1]湖南大学研究生院隆平分院,长沙410125 [2]湖南省农业科学院植物保护研究所,长沙410125 [3]湖南省农业科学院蔬菜研究所,长沙410125

出　　处：《昆虫学报》2022年第8期1018-1025,共8页Acta Entomologica Sinica

基　　金：国家自然科学基金项目(32030088,32072383,31901854);湖南省自然科学基金项目(2019 JJ30014);大宗蔬菜产业体系项目(CARS-16-E-17,CARS-23-D-02)。

摘　　要：【目的】由粉虱传播的番茄褪绿病毒(tomato chlorosis virus,ToCV)具有较强的暴发性和流行性,目前该病毒现已蔓延至世界各地,可为害多种作物,对农林经济生产造成严重危害。病毒侵染会影响介体昆虫的寄主选择性和取食行为,从而影响病毒传播。本研究旨在明确ToCV对MED烟粉虱Bemisia tabaci在不同寄主上的寄主选择性及取食行为的影响。【方法】利用Y型嗅觉仪测定携带和未携带ToCV的MED烟粉虱雌成虫的寄主选择性,利用刺吸电位(electrical penetration graph,EPG)技术比较携带和未携带ToCV的烟粉虱雌成虫在健康的番茄、辣椒、棉花和豇豆4种寄主植株上的取食行为差异。【结果】未携带ToCV的MED型烟粉虱雌成虫对于4种寄主植物的选择偏好性排序为番茄、辣椒>棉花>豇豆;携带ToCV烟粉虱雌成虫对4种寄主植物的选择性偏好性降低,排序为番茄、辣椒、棉花>豇豆。与未携带ToCV烟粉虱雌成虫相比,携带ToCV烟粉虱雌成虫取食4种植物所产生的刺探次数均显著增加,第1次到达韧皮部的时间有明显延后,取食总时间与韧皮部的取食时间明显减少。【结论】ToCV使烟粉虱对寄主的选择偏好性和取食行为发生显著改变,从而增加了病毒在寄主植物间传播的可能。【Aim】Tomato chlorosis virus(ToCV)is transmitted by whiteflies and breaks out heavily.At present,ToCV has spread to most regions of the world,and can harm a variety of crops and cause serious damage to agricultural and forestry economic production.Virus infection can influence the host preference and feeding behaviors of vector insects,thus influencing virus transmission.The aim of this study is to investigate the effects of ToCV on the host preference and feeding behaviors of Bemisia tabaci MED adults on different host plants.【Methods】The host selectivity of ToCV-infected and non-infected B.tabaci MED female adults was determined by Y-tube olfactometer,and the feeding behaviors between ToCV-infected and ToCV-non-infected female adults of B.tabaci MED on four healthy host plants including tomato,pepper,cotton and cowpea were compared with electrical penetration graph(EPG).【Results】The preference of ToCV-non-infected female adults of B.tabaci MED to tomato and pepper was the highest,that to cotton followed and that to cowpea was the least.The preference of female adults of B.tabaci MED infected by ToCV to tomato,pepper and cotton was higher than that to cowpea.The numbers of probes of the ToCV-infected female adults of B.tabaci MED on the four plants were significantly increased,their first time to probe phloem was significantly delayed,and their total feeding time and phloem feeding time significantly decreased as compared with those of the ToCV-non-infected female adults.【Conclusion】ToCV significantly changes the host preference and feeding behaviors of B.tabaci,thus increases the probability of its own transmission among host plants.

关 键 词：番茄褪绿病毒 MED烟粉虱 寄主选择性 取食行为 刺吸电位 

分 类 号：Q965.8[生物学—昆虫学]