一种检测羧酸酯酶的Turn-off型荧光探针的制备  

Design and synthesis of a turn-off fluorescent probe for the detection of carboxylesterase

作　　者：谢国旺 滕明刚 游辉 刘家芳 柴慧芳 XIE Guowang;TENG Minggang;YOU Hui;LIU Jiafang;CHAI Huifang(School of Pharmacy,Guizhou University of Traditional Chinese Medicine,Guiyang,Guizhou 550025,China)

机构地区：[1]贵州中医药大学药学院,贵州贵阳550025

出　　处：《江西化工》2022年第4期93-97,共5页Jiangxi Chemical Industry

基　　金：贵州省科技计划项目(项目编号:黔科合基础-ZK[2022]一般455);贵州省科技计划项目(项目编号:黔科合基础-ZK[2022]一般489);贵州省2022年度省科技厅基础研究计划(项目编号:2040-040220035)。

摘　　要：羧酸酯酶(CES)是一种α/β-丝氨酸水解酶,能通过水解酯或酰胺来代谢药物(如卡培他滨)和农药(如拟除虫菊酯),或者是代谢内源性酯(如胆固醇酯)。本研究基于咔唑为原料,制备一种turn-off荧光探针TX-01用于检测羧酸酯酶,并通过探针分子与羧酸酯酶反应前后的荧光强度变化检测该酶的活性。该有机小分子荧光探针的最大吸收波长为423 nm,最大发射波长为580 nm,斯托克斯位移为157 nm,其与羧酸酯酶反应前呈橙黄色荧光,反应后荧光淬灭。Carboxylesterase( CES) is an α,β-serine hydrolase which can hydrolyze esters or amides to metabolize drugs,pesticides,or metabolize endogenous esters,for example,Capecitabine,pyrethroids,and cholesterol esters respectively.Here,a turn-off fluorescent probe TX-01 using carbazole as a raw material was prepared for the detection of carboxylesterase. The probe exhibited orange-yellow fluorescence before the reaction with the carboxylesterase,and it was quenched after the reaction. The maximum absorption wavelength is 423 nm,the maximum emission wavelength is 580 nm,and the Stokes shift is 157 nm.

关 键 词：羧酸酯酶 荧光探针 酶检测 咔唑检测 

分 类 号：O657.3[理学—分析化学]