40份山西黍稷资源抗旱性鉴定  

Drought Resistance Identification of 40 Broomcorn Millet Resources in Shanxi Province

作　　者：王杨 谭梦玉 王海岗[1,2] 陈凌[2] 王瑞云[1,2] 乔治军 WANG Yang;TAN Mengyu;WANG Haigang;CHEN Ling;WANG Ruiyun;QIAO Zhijun(College of Agriculture,Shanxi Agricultural University,Taigu 030801,China;Center for Agricultural Genetic Resources Research,Shanxi Agricultural University/Key Laboratory of Crop Gene Resources and Germplasm Enhancement on Loess Plateau,Ministry of Agriculture and Rural Affairs/Shanxi Key Laboratory of Genetic Resources and Genetic Improvement of Minor Crops,Taiyuan 030031,China)

机构地区：[1]山西农业大学农学院,山西太谷030801 [2]山西农业大学农业基因资源研究中心/农业农村部黄土高原作物基因资源与种质创制重点实验室/杂粮种质资源发掘与遗传改良山西省重点实验室,山西太原030031

出　　处：《山西农业科学》2022年第9期1261-1268,共8页Journal of Shanxi Agricultural Sciences

基　　金：国家自然科学基金项目(31271791);现代农业产业技术体系建设专项资金(CARS-06-14.5-A16);山西省自然科学基金项目(201901D11126)。

摘　　要：为了解山西黍稷种质资源的抗旱性,以筛选抗旱性较强的品种,为科学、规范地建立糜子抗旱性鉴定标准和研究体系提供科学依据,将40个山西黍稷的核心资源在室内用PEG6000进行模拟干旱胁迫处理,应用多种分析方法对发芽率、苗长、根长、苗鲜质量、根鲜质量、苗干质量、根干质量、叶绿素含量等各指标进行比较分析,并对品种进行综合评价和分类。结果表明,通过对芽期和苗期抗旱性指标的测定发现,PEG6000胁迫下除根长较对照增大0.071%外,其他7个指标的测量值较对照均有所下降,发芽率下降幅度高达95.262%,且第3天测定时试验组均未发芽,可知干旱胁迫对芽苗期均有抑制作用,发芽率对干旱胁迫最敏感;相关性分析显示,各指标间相关性多数呈显著或极显著,说明PEG胁迫对其作用明显;主成分分析表明,相对苗长、相对苗鲜质量、相对叶绿素含量可作为评价苗期抗旱性的重要指标;采用平均值-标准差逐级分级法和聚类分析法综合鉴定芽、苗期抗旱性,结果筛选出芽期极强抗旱类型和强抗旱类型各1份,苗期抗旱性分析中筛选出8份极强抗旱类型和5份强抗旱类型。综合分析,来自繁峙的紫秆黍(00003289)在芽苗期均为极强抗旱类型,且晋南地区品种抗旱性较强,可以为黍稷育种中亲本的选配提供优异的供体来源。In order to understand the drought resistance of broomcorn millet germplasm resources in Shanxi,and screen out the varieties with stronger drought resistance to provide scientific basis for scientific and normative establishment of the drought resistance identification standard and research system of broomcorn millet.In this study,the core resources of 40 broomcorn millets in Shanxi were treated with PEG6000 to simulate drought stress in laboratory,and various analysis methods were used to compare and analyze the indexes including germination rates,seedling length,root length,seedling fresh weight,root fresh weight,seedling dry weight,root dry weight,chlorophyll content and comprehensively evaluate and classify the varieties.Through the measurement of drought resistance indexes at the budding stage and seedling stage,it was found that except the root length increaesd by 0.071%compared with that in the control under PEG6000 stress,the measured values of the other 7 indexes decreased compared with those in the control,and the experimental group did not germinate on the 3rd day,indicating that drought stress had an inhibitory effect on the budding stage and seedling stage,and the germination rate was the most sensitive to drought stress.Correlation analysis showed that most of the correlations between the indexes were significant or extremely significant,indicating that PEG stress had an obvious effect on them.The principal component analysis showed that the relative seedling length,relative seedling fresh weight,and relative chlorophyll content could be used as the important indexes to evaluate the drought resistance at seedling stage.The mean-standard deviation step-by-step grading method and cluster analysis method were used to comprehensively identify the drought resistance at budding stage and seedling stage,and screened out one extremly strong drought resistance type and one strong drought resistance type at budding stage,eight extremly strong drought resistance type and five strong drought resistance types at

关 键 词：黍稷 抗旱鉴定 PEG6000 芽期 苗期 

分 类 号：S516[农业科学—作物学]