Multi-tissue scRNA-seq reveals immune cell landscape of turbot (Scophthalmus maximus)  

作　　者：Weijie Chen Jianchang Huang Wei Wang Ying Wang Hao Chen Qiyao Wang Yuanxing Zhang Qin Liu Dahai Yang 

机构地区：[1]State Key Laboratory of Bioreactor Enginering East China University of Science and Technology,Shanghai 200237,China [2]Southemn Marine Science and Engineering Guangdong Laboratory(Zhuhai),Zhuhai 519000,China [3]CLaboratoy for Marine Biology and Biotehnology,Qingdao National Laboratory for Marine Science and Technology,Qingdao,266071,China [4]Shanghai Enginering Research Center of Mariculured Animal Vaccines,Shanghai 200237,China

出　　处：《Fundamental Research》2022年第4期550-561,共12页自然科学基础研究（英文版）

基　　金：supported by the National Natural Science Foundation of China[Grant Nos.32025038(Q.L.),32122090(D.Y.)];and the Natural Science Foundation of Shanghai[Grant No.20ZR1415500(D.Y.)].

摘　　要：In vertebrates,bony fishes possess not only innate immune cells but also T and B cells that are equivalent to those in mammals.However,the precise sub-cluster of immune cells in teleost fish remains largely unknown.Herein,we developed a dynamic bacterial infection model in turbot(Scophthalmus maximus)and created a fish immune cell landscape(FICL)for a primary lymphoid organ(head kidney),a secondary lymphoid organ(spleen),and barrier tissues(gill and posterior intestine).Moreover,through comprehensive characterization of the expression profiles of 16 clusters,including dendritic cells-like(DCs-like),macrophages(Mos),neutrophils,NK cells,as well as 12 sub-clusters of T and B cells,we found that CD8+CTLs,CD4-CD8-T,Th17 and ILC3-2 like cells posssa bifunctional role associated with cytotoxicity and immunoregulation during bacterial infection.To our knowledge,these results could provide a useful resource for a better understanding of immune cells in teleost fish and could act as a comprehensive knowledge base for assessing the evolutionary mechanism of adaptive immunity in vertebrates.

关 键 词：ScRNA-Seq Fish immune cellandscape Dynamic immunecell responses Edwardsiella piscicida infection TURBOT 

分 类 号：Q953[生物学—动物学]