水稻抽穗期调控基因Hd6的PARMS标记开发与利用  

Development and Application of PARMS Markers Specific for Rice Heading Date Regulation Gene Hd6

作　　者：彭永彬 杜晨阳 郑崇珂 周晋军[1] 孙伟[1] 和亚男 谢先芝[1] Peng Yongbin;Du Chenyang;Zheng Chongke;Zhou Jinjun;Sun Wei;He Yanan;Xie Xianzhi(Institute of Wetland Agriculture and Ecology,Shandong Academy of Agricultural Sciences,Jinan 250100,China;College of Life Sciences,Shandong Agricultural University,Taian 271018,China)

机构地区：[1]山东省农业科学院湿地农业与生态研究所,山东济南250100 [2]山东农业大学生命科学学院,山东泰安271018

出　　处：《山东农业科学》2022年第8期1-6,共6页Shandong Agricultural Sciences

基　　金：山东省自然科学基金青年项目(ZR2020QC112)。

摘　　要：水稻抽穗期调控基因Hd6有Hd6^(Nip)和Hd6^(Kasa)两个等位基因型,其中有功能的Hd6^(Kasa)型在长日照条件下抑制抽穗,可有效应用于水稻育种工作。为提高Hd6的选择效率,本研究根据水稻品种‘Kasalath’与‘日本晴’在Hd6第436位密码子上的A/T变异,基于五引物扩增受阻突变体系(penta-primer amplification refractory mutation system,PARMS)标记技术原理,开发了特异性PARMS标记Hd6^(Kasa)-PARMS,并利用该标记对‘蜀恢498’与‘苏秀867’的回交BC_(2)F_(2)群体进行Hd6等位基因的分型,成功分出Hd6^(Nip)型、Hd6^(Kasa)型及对应的杂合型,经进一步测序验证,基因分型的准确度达到100%;对基因分型与抽穗期表型的相关性进行Pearson相关分析,相关系数为0.858,达到极显著相关水平。综上,利用该标记可快速有效鉴定出水稻抽穗期调控基因Hd6的等位基因分型,从杂交后代群体中分离出携带Hd6^(Kasa)基因型的单株,对提高育种效率、促进水稻生育期的遗传改良具有重要应用价值。Hd6 is a quantitative trait locus involved in rice photoperiod sensitivity,including Hd6^(Nip) and Hd6^(Kasa) alleles.Hd6^(Kasa) allele greatly inhibites days-to-heading by long day(LD) conditions,so it is useful for breeding of rice cultivars.In order to improve the selection efficiency,a PARMS marker Hd6^(Kasa)-PARMS was developed based on the variation of A/T at the 436 th codon of Hd6 gene between the indica variety Kasalath and the japonica variety Nipponbare.The BC_(2)F_(2) population constructed with Shuhui 498 as donor parent and Suxiu 867 as recurrent parent.The developed Hd6^(Kasa)-PARMS marker could accurately distinguish Hd6^(Nip),Hd6^(Kasa) and hybrid alleles with the accuracy as 100% through verified by sequence analysis of DNA samples.The Pearson correlation analysis showed that the PARMS marker genotyping results were highly correlated with their heading date phenotypes.In conclusion,using the developed Hd6^(Kasa)-PARMS marker could rapidly and effectively identify the allele genotype of Hd6,and isolate individual plants carrying Hd6^(Kasa) allele,which has an important application value in increasing selection efficiency and promoting genetic improvement.

关 键 词：水稻 抽穗期 Hd6 PARMS标记 等位基因分型 

分 类 号：S511[农业科学—作物学] Q781[生物学—分子生物学]