干旱胁迫和盐胁迫对紫花苜蓿种子萌发的影响  

Effects of drought stress and salt stress on Medicago sativa seed germination

作　　者：鲁富宽[1] 宋雪峰 胡云[1] 王润莲[1] 荣磊 黄修梅[1] LU Fukuan;SONG Xuefeng;HU Yun;WANG Runlian;RONG Lei;HUANG Xiumei(College of Vocational and Technical,Inner Mongolia Agricultural University,Baotou 014100,China;Yuantaifeng(Baotou)Biotechnology Co.,Ltd.,Baotou 014100,China)

机构地区：[1]内蒙古农业大学职业技术学院,内蒙古包头014100 [2]元泰丰(包头)生物科技有限公司,内蒙古包头014100

出　　处：《黑龙江畜牧兽医》2022年第16期102-107,共6页Heilongjiang Animal Science And veterinary Medicine

基　　金：元泰丰校企合作横向联合项目。

摘　　要：为了对比紫花苜蓿种子萌发对干旱胁迫和盐胁迫的耐受能力,试验分别利用不同浓度的聚乙二醇(PEG-6000)溶液和NaCl溶液模拟干旱胁迫和盐胁迫,形成渗透势分别为-0.24,-0.49,-0.74,-0.99,-1.23,-1.48 MPa的等渗环境,并设置对照处理(渗透势为0),研究紫花苜蓿不同渗透势下的干旱胁迫和盐胁迫对种子萌发情况及胁迫解除后的种子复萌情况的影响。结果表明:两种胁迫处理均导致苜蓿种子初始萌发时间推迟,在高渗环境中,干旱胁迫处理的苜蓿种子初始萌发时间要长于盐胁迫处理;随着渗透势的降低,两种胁迫处理的种子萌发持续时间、种子萌发率和种子萌发指数均大体呈现下降趋势,种子胚根长度均先保持不变后呈现下降趋势,种子萌发相对迫害率均呈现上升趋势,种子复萌率大体呈现先上升后下降的趋势。在渗透势相同时,干旱胁迫处理的种子萌发率、种子萌发指数均低于盐胁迫,种子萌发相对迫害率均高于盐胁迫。在低渗环境中,干旱胁迫处理的种子复萌率明显高于盐胁迫;与对照处理比较,当渗透势为-0.74~-0.24 MPa时,干旱胁迫处理的种子胚根长度略有降低,但差异不显著(P>0.05);当渗透势为-0.24 MPa时,盐胁迫处理的种子萌发率略有降低,但差异不显著(P>0.05);当渗透势为-0.24 MPa和-0.49 MPa时,盐胁迫处理的种子胚根长度略有降低,但差异不显著(P>0.05)。说明苜蓿种子萌发对盐胁迫的耐受性要强于干旱胁迫,且在低渗环境下,苜蓿种子萌发对盐胁迫有一定的耐受性,胚根生长对干旱胁迫和盐胁迫均有一定的耐受性。In order to compare the tolerance of Medicago sativa seed germination to drought stress and salt stress,in this experiment,different concentrations of PEG-6000 solution and NaCl solution were used to simulate drought stress and salt stress,forming an isotonic potential of-0.24,-0.49,-0.74,-0.99,-1.23,-1.48 MPa,respectively,and a control treatment isotonic potential was(0)set at the same time.The effects of drought stress and salt stress on alfalfa seed germination and seed re-germination after stress relief were examined.The results showed both stress treatments delayed the initial germination time of Medicago sativa seeds,and the initial germination time of alfalfa seeds under drought stress was longer than that under salt stress in the hypertonic solution.With the decrease of osmotic potential,the duration of seed germination,seed germination rate and seed germination index showed a downward trend under the two stress treatment;the radicle growth firstly maintain a certain length and then showed a downward trend;the relative germination rate of seed germination showed an upward trend,and the re-germination rate generally showed a first upward and then downward trend.Under the same osmotic solution,the seed germination rate and seed germination index under drought stress treatment were lower than those under salt stress,and the relative persecution rate was higher than that under salt stress.In low osmotic solution,the re-germination rate of seeds under drought stress was significantly higher than that under salt stress.Compared with the control treatment,when the osmotic potential was-0.74--0.24 MPa,the radicle length of seeds under drought stress decreased slightly,but the difference was not significant(P>0.05);when the osmotic potential was-0.24 MPa,the seed germination rate under salt stress treatment decreased slightly,but the difference was not significant(P>0.05);when the osmotic potential was-0.24 MPa and-0.49 MPa,the radicle length of seeds under salt stress decreased slightly,but the difference was not

关 键 词：紫花苜蓿 干旱胁迫 盐胁迫 种子萌发率 复萌率 

分 类 号：S816.5[农业科学—饲料科学]