吴江黄叶香青菜结荚期不同部位叶绿素荧光特征比较  

Comparison of Chlorophyll Fluorescence Characteristics in Different Parts of Wujiang Yellow-Leafed Brassica chinensis at Pod Setting Stage

作　　者：陶菊红 李亚娟 张涛[3,4] TAO Juhong;LI Yajuan;ZHANG Tao(Changshu Institute of Agriculture Science,Changshu 215500;Changshu Seed Management Station,Changshu 215500;School of Biology and Food Engineering,Changshu Institute of Technology,Changshu 215500,China;Changshu Institute of Technology-Duanmu Yinxi Rice Breeding Research and Promotion Center,Changshu Institute of Technology,Changshu 215500,China)

机构地区：[1]常熟市农业科学研究所,江苏常熟215500 [2]常熟市种子管理站,江苏常熟215500 [3]常熟理工学院生物与食品工程学院,江苏常熟215500 [4]常熟理工学院常熟理工-端木银熙水稻育种研究推广中心,江苏常熟215500

出　　处：《常熟理工学院学报》2022年第5期79-82,90,共5页Journal of Changshu Institute of Technology

基　　金：常熟市科技发展计划项目“苏州地方香青菜种质资源保护及开发利用”(CN201911)。

摘　　要：本文以常熟引种的吴江黄叶香青菜为材料,通过对结荚期基部叶片、顶端叶片以及长角果荚的叶绿素荧光参数进行测定,研究了该种质发育期光合生理特征.结果表明:结荚期吴江黄叶香青菜基部莲座叶片、上部披针叶片和长角果荚最大量子效率(Fv/F_(m))均处于0.83左右;叶片及长角果荚的实际量子效率(ΔF/F_(m)ˊ)日变化均随光照时间的延长逐步下降,到傍晚17点有所上升,但是上午时间段基部莲座叶片ΔF/F_(m)ˊ要高于上部披针叶片和长角果荚.所有测定部位均表现出典型的快速光曲线特征,其中基部莲座叶片初始斜率(α)要高于顶部披针叶片,但顶部披针叶片半饱和光强(Ik)要高于其他部位;最大相对电子传递速率不同部位未表现出显著差异.对快速叶绿素荧光诱导动力学参数的测定显示:不同部位叶片和长角果荚在单位反应中心吸收光能(ABS/RC)、用于还原Q_(A)的能量(TRo/RC)、用于电子传递的能量(ETo/RC)以及耗散的能量(DIo/RC)均未表现出明显差异.这些结果说明在吴江黄叶香青菜的结荚期,基部莲座叶片、顶部披针叶片和长角果荚均表现出活跃的光合生理活性,尤其是长角果荚的光合特性可能与籽粒的成熟密切相关.本研究从光能利用角度探讨了吴江黄叶香青菜在结荚期的光合生理特征,为今后该种质在常熟的栽培和应用提供了数据支撑.In this study,we used the Wujiang B.chinensis cultivated in Changshu as the material and identified its photosynthetic physiological characteristics in Changshu by measuring the chlorophyll fluorescence parameters of basal leaves,upper leaves and pods at the pod setting stage.The results indicated that the maximum quantum efficiency(F_(v)/F_(m))of all parts was about 0.83;and that their actual quantum efficiency(ΔF/F_(m)ˊ)declined after sunrise and rose at 17:00.However,theΔF/F_(m)ˊof the basal leaves was higher than that of the uppers and the pods during the morning.All the measured parts showed that there were typical rapid light curve characteristics,and that the initial slopes(α)of the basal leaves were higher than those of the uppers,but the semi-saturation light intensity(I_(K))of the upper leaves was higher than that of the others.No significant differences were revealed about the maximum relative electron transfer rate among the three parts.According to the results from the fast chlorophyll fluorescence induction kinetic parameters,calculated from per unit reaction center of leaves and pods,the value of the absorbed light energy(ABS/RC),the energy used to reduce Q_(A)(TRO/RC),the energy used to make electron transfer(ETO/RC)and the dissipated energy(DIO/RC),showed no significant differences.These results indicated that at the pod setting stage of B.chinensis,the basal leaves,the upper leaves and the pods all showed active photosynthetic physiological activities,especially the findings about the pods which might relate to the maturity of grains.Thus,this study provided experimental data support for the cultivation and application of Wujiang B.chinensis in Changshu in the future by exploring its photosynthetic physiological characteristics at the pod setting stage from the perspective of light energy utilization.

关 键 词：香青菜 光合生理 叶绿素荧光 

分 类 号：S635[农业科学—蔬菜学]