微量元素对合并糖脂代谢异常子痫前期患者内皮祖细胞增殖及迁移活性的影响  

Effects of trace elements for treating endothelial progenitor cells of pregnant women with preeclampsia and abnormal glucose and lipid metabolism on the proliferation and migration activity of these cells

作　　者：王央玲 黄海桃 符火[1] WANG Yangling;HUANG Haitao;FU Huo(The First Affiliated Hospital of Hainan Medical College,Haikou,Hainan Province,570102)

机构地区：[1]海南医学院第一附属医院,海口570102

出　　处：《中国计划生育学杂志》2022年第9期1950-1954,共5页Chinese Journal of Family Planning

基　　金：海南省重点研发计划项目(ZDYF2019162)。

摘　　要：目的:分析微量元素对合并糖脂代谢异常子痫前期患者内皮祖细胞(EPCs)细胞增殖及迁移活性的影响。方法:收集2018年11月-2021年11月在本院就诊的20例合并糖脂代谢异常的子痫前期患者晨空腹静脉血,分离培养EPCs,分别采用MTT比色法、Transwell小室检测不同剂量的微量元素对EPCs细胞增殖及迁移活性的影响,采用RT-PCR、Western blot法测定不同剂量微量元素对Caspase-3 mRNA、Caspase-3蛋白表达量的影响。结果:随着微量元素剂量减少EPCs细胞逐渐降低,即光密度值、迁移细胞数分别为微量元素6ml组(2.06±0.09、38.71±4.59/cells×200)>4 ml组(1.70±0.10、29.47±3.52/cells×200)>2 ml组(1.52±0.09、25.47±4.62/cells×200)>对照组(1.33±0.04、17.88±2.75/cells×200);Caspase-3 mRNA、Caspase-3蛋白表达量升高,即微量元素6ml组(0.217±0.05caspase-3/GAPHD、0.212±0.03)<4 ml(0.537±0.08caspase-3/GAPHD、0.427±0.04)<2 ml(0.669±0.09caspase-3/GAPHD、0.617±0.05)<对照组(0.880±0.06caspase-3/GAPHD、0.749±0.04)(均P<0.05)。结论:微量元素可促进合并糖脂代谢异常子痫前期患者EPCs细胞增殖及提升其迁移活性,下调Caspase-3 mRNA及Caspase-3蛋白表达量。Objective:To analyze the effects of trace elements for treating endothelial progenitor cells(EPCs)of pregnant women with preeclampsia and abnormal glucose and lipid metabolism on the proliferation and migration activity of these EPCs.Methods:The morning fasting venous blood samples were collected from 20 pregnant women with preeclampsia and abnormal glucose and lipid metabolism between November 2018 and November 2021 and the EPCs in these blood samples were isolated and cultured.The effects of different dose of trace elements on the proliferation and migration activity of EPCs were detected by MTT colorimetry and Transwell chamber technology.The effects of different doses of trace elements on the expression levels of Caspase-3 mRNA and Caspase-3 protein of EPCs were determined by RT-PCR and Western blot.Results:As the dose of trace elements had decreased,the optical density and the number of migrating cells of EPCs in trace elements 6 ml group(2.06±0.09,38.71±4.59/cells×200),in trace elements 4 ml group(1.70±0.10,29.47±3.52/cells×200),in trace elements 2 ml group(1.52±0.09,25.47±4.62/cells×200),and in control group(1.33±0.04,17.88±2.75/cells×200),which had decreased gradually.As the dose of trace elements had decreased,the expressions of caspase-3 mRNA and caspase-3 protein of EPCs in trace elements 6 ml group(0.217±0.05caspase-3/GAPHD,0.212±0.03),in trace elements 4 ml group(0.537±0.08caspase-3/GAPHD,0.427±0.04),in trace elements 2 ml group(0.669±0.09caspase-3/GAPHD,0.617±0.05),and in control group(0.880±0.06caspase-3/GAPHD,0.749±0.04)had increased gradually(all P<0.05).Conclusion:Trace elements may promote the proliferation and enhance the migration activity of the EPCs of pregnant women with preeclampsia and abnormal glucose and lipid metabolism,and which can down regulate the expression quantities of Caspase-3 mRNA and Caspase-3 protein of the EPCs.

关 键 词：子痫前期 糖脂代谢异常 微量元素 内皮祖细胞增殖 迁移活性 CASPASE-3 

分 类 号：R714.244[医药卫生—妇产科学]