顶空气相色谱-质谱法同时测定尿中5种有机溶剂接触的生物监测指标  

Simultaneous determination of five biological monitoring indicators of organic solvents in urine by head-space gas chromatography-mass spectrometry

作　　者：刘亚攀 宋为丽 王敏 杨莉 张成 LIU Ya-pan;SONG Wei-li;WANG Min;YANG Li;ZHANG Cheng(Wuhan Prevention and Treatment Center for Occupational Diseases,Wuhan,Hubei 430015,China)

机构地区：[1]武汉市职业病防治院卫生检验科,湖北武汉430015

出　　处：《现代预防医学》2022年第15期2826-2829,共4页Modern Preventive Medicine

基　　金：武汉市卫生健康委员会科研基金资助(WG18Q07);武汉科技大学职业危害识别与控制湖北省重点实验室开放基金项目(OHIC2020Z04)。

摘　　要：目的建立尿中丙酮、二氯甲烷、甲乙酮、1-溴丙烷、甲基异丁基甲酮的顶空气相色谱-质谱测定方法。方法尿样经顶空自动进样器进样,气相色谱柱分离,质谱检测器检测。结果本方法测定尿中各组分在各浓度范围内线性良好,相关系数为0.9991~0.9996,各组分检出限分别为0.40 mg/L、0.60μg/L、0.02 mg/L、0.09μg/L、0.01 mg/L,方法精密度为1.8%~4.9%,样品加标回收率为96.9%~104.5%。结论本方法检出限低,准确度、精密度高,样品前处理简单,适用于尿中该5种生物监测指标的同时测定。Objective To establish a head-space gas chromatography-mass spectrometry(GC-MS)for the determination of acetone,dichloromethane,methyl ethyl ketone,1-bromopropane,and methyl isobutyl ketone in urine.Methods Head-space gases of urine samples were injected into GC by automatic headspace sampler and detected by mass spectrometer.Results Within the linear ranges,the correlation coefficients were between 0.9991 and 0.9996.The detection limits were 0.4 mg/L,0.6μg/L,0.02 mg/L,0.09μg/L,0.01 mg/L.The precisions were between 1.8%and 4.9%,and the recovery rates were between 96.9%and 104.5%.Conclusion The method has low detection limits,high accuracy,high precision and simple pretreatment,which is suitable for the determination of the 5 biological monitoring indicators in urine.

关 键 词：顶空法 气相色谱-质谱法 尿 有机溶剂 生物监测指标 

分 类 号：R115[医药卫生—公共卫生与预防医学]