纳米孔测序在血流感染中的应用  

Application of nanopore sequencing in bloodstream infection

作　　者：吴泽刚[1] 顾剑[2] 郑红云[1] 徐万洲[2] 肖红军 童永清[1,2] WU Zegang;GU Jian;ZHENG Hongyun;XU Wanzhou;XIAO Hongjun;TONG Yongqing(Department of Clinical Laboratory,Renmin Hospital of Wuhan University,Wuhan,Hubei 430060,P.R.China;Clinical Molecular Diagnostics of Wuhan University,Wuhan,Hubei 430060,P.R.China;Medical Vocational and Technical School of Wuhan University,Wuhan,Hubei 430060,P.R.China)

机构地区：[1]武汉大学人民医院检验医学中心,武汉430060 [2]武汉大学临床分子诊断中心,武汉430060 [3]武汉大学医学职业技术学院,武汉430060

出　　处：《华西医学》2022年第8期1166-1169,共4页West China Medical Journal

摘　　要：目的评估纳米孔测序的基本性能及临床应用价值,以期为临床病原学的快速检测提供新思路。方法收集2021年12月-2022年5月武汉大学人民医院疑似血流感染的住院患者血标本,利用纳米孔测序和血培养的方法同时对入选患者血标本的致病菌进行鉴定,并对纳米孔测序的基本性能和临床应用价值进行评估。结果共纳入251例患者,纳米孔测序法检出病原菌119例(47.4%),检出率高于血培养法的23例(9.2%)(χ^(2)=79.167,P<0.001)。对比两种方法结果不具有一致性(kappa=0.052,P=0.175)。纳米孔测序存在一定漏检率。在检出病原菌的种类上,纳米孔测序法检出的细菌有47种,真菌15种。结论纳米孔测序较血培养病原检出率高且检出病原种类多,该技术在血流感染的病原体快速诊断中优势明显。Objective To evaluate the basic performance and clinical application value of nanopore sequencing,in order to provide new ideas for the rapid detection of clinical etiology.Methods From December 2021 to May 2022,blood samples from inpatients suspected of bloodstream infection in Renmin Hospital of Wuhan University were collected,and the nanopore sequencing platform and blood culture method were used to simultaneously identify the pathogenic bacteria in the blood samples of the selected patients,and identify the pathogenic bacteria in the blood samples of the selected patients.The basic performance and clinical utility of nanopore sequencing were evaluated.Results A total of 251 patients were included,and 119 patients(47.4%)were found to have pathogens by nanopore sequencing,which was higher than that of 23 patients(9.2%)by blood culture(χ^(2)=79.167,P<0.001).The results of the two methods are not consistent(kappa=0.052,P=0.175).Nanopore sequencing has a certain missed detection rate.In terms of the types of pathogenic bacteria detected,47 bacteria and 15 fungi were detected by nanopore sequencing.Conclusion Compared with blood culture,nanopore sequencing has a higher detection rate and more types of pathogens.This technology has obvious advantages in the rapid diagnosis of bloodstream infection pathogens.

关 键 词：血流感染 病原体 纳米孔测序 血培养 

分 类 号：R446.5[医药卫生—诊断学]