巯基化氧化石墨烯光化学传感器的制备及对痕量Hg^(2+)的检测  

Preparation of Photochemical Sensor of Thiol-Modified Graphene Oxide for Determination of Trace Hg^(2+)

作　　者：杨磊 林潇雨 朱智飞 郭子建 蔡力锋 傅丽君 王海燕 陈伟杰 原瑞芬 Yang Lei;Lin Xiaoyu;Zhu Zhifei;Guo Zijian;Cai Lifeng;Fu Lijun;Wang Haiyan;Chen Weijie;Yuan Ruifen(College of Environmental and Biological Engineering,Fujian Provincial Key Laboratory of Ecologytoxicological Effects&Control for Emerging Contaminants,Putian University,Putian,Fujian 351100,China;College of Life Science,Fujian Agriculte and Forestry University,Fuzhou,Fujian 350002,China;Putian Agricultural Environmental protection and Energy Station,Putian,Fujian 351100,China)

机构地区：[1]莆田学院环境与生物工程学院福建省新型污染物生态毒理效应与控制重点实验室,福建莆田351100 [2]福建农林大学生命科学院,福建福州350002 [3]莆田市农业环保能源站,福建莆田351100

出　　处：《化学世界》2022年第4期208-215,共8页Chemical World

基　　金：国家自然科学青年基金(Nos.31400318,21206079);福建省科技厅高校产学合作及引导性(Nos.2015H6017,2020Y0089);福建省教育厅中青年教师教育科研(No.JAT200526);莆田市科技计划(Nos.2018G2001,2017G2012,2017R4001);莆田学院校基金(Nos.2016080,PY16012);大学生创新创业训练计划(Nos.201911498031,202011498002,202011498005)资助项目。

摘　　要：为拓宽氧化石墨烯(GO)光化学传感检测金属离子的方法,以石墨和巯基乙醇为主要原料制备了高荧光强度巯基化氧化石墨烯(GO-SH)。红外、紫外和XRD分析表明成功地制备了片层厚度更薄的巯基化GO,GO-SH的荧光强度高于GO,且其仅在502.9nm处存在单一荧光峰。通过分子荧光光谱仪研究了GO-SH与金属离子的相互作用行为,结果表明,GO-SH对Hg^(2+)具有单一识别性;Hg^(2+)对GO-SH荧光有显著猝灭作用且使GO-SH光谱峰发生显著位移。建立了灵敏度高、选择性好的GO-SH荧光光化学传感检测Hg^(2+)的方法,在Hg^(2+)浓度为5×10^(-4)~5×10^(-7) mol·L^(-1),其线性方程为:y=81.05502 x+4.8508,其中判定系数(R^(2))=0.998,检出限为1.840×10^(-8) mol·L^(-1)。GO-SH对实际水样中Hg^(2+)检测回收率为99.834%~100.030%,相对标准偏差为0.631~0.718,表明其具有较好的应用效果。To broaden the photochemical detection method of graphene oxide(GO)to metal ion,thiol-modified graphene oxide(GO-SH)composite with high fluorescence intensity was prepared by using graphite and mercapto alcohol as raw materials.Infrared,UV/Vis spectrscopy and X-ray diffraction pattern analysis showed that GO-SH with small lamellar thickness was prepared successfully,and the fluorescence intensity of GO-SH was higher than that of GO,and it had a single fluorescence peak only at 502.9 nm.The interaction between GO-SH and metal ions was studied by molecular fluorescence spectrometer.The results showed that GO-SH had a single identity to Hg^(2+),and Hg^(2+)had a significant quenching effect on the fluorescence of GO-SH and made the peak shift significantly.Finally,a new method of GO-SH fluorescence photochemical detection to Hg^(2+)with high sensitivity and selectivity was established.When Hg^(2+)concentration was 5×10^(-4)~5×10^(-7) mol·L^(-1),its linear equation was:y=81.05502x+4.8508,in which determination coefficient(R^(2))=0.99835 and the detection limit was 1.54×10^(-12) mol·L^(-1).The percent recovery of Hg^(2+)in aqueous samples by GO-SH was in the range of 99.834%~100.030%,and the relative standard deviation was 0.631-0.718,which indicated that it had a good application.

关 键 词：巯基化氧化石墨烯 荧光传感 识别 Hg^(2+) 

分 类 号：O657.319[理学—分析化学] X830.2[理学—化学]