CRISPR-Cas12a-Empowered Electrochemical Biosensor for Rapid and Ultrasensitive Detection of SARS-CoV-2 Delta Variant  

作　　者：Chenshuo Wu Zhi Chen Chaozhou Li Yabin Hao Yuxuan Tang Yuxuan Yuan Luxiao Chai Taojian Fan Jiangtian Yu Xiaopeng Ma Omar AAl-Hartomy SWageh Abdullah GAl-Sehemi Zhiguang Luo Yaqing He Jingfeng Li Zhongjian Xie Han Zhang 

机构地区：[1]International Collaborative Laboratory of 2D,Materials for Optoelectronics Science and Technology of Ministry of Education,Institute of Microscale Optoelectronics,College of Physics and Optoelectronic Engineering,Shenzhen University,Shenzhen 518060,People’s Republic of China [2]Shenzhen International Institute for Biomedical Research,Shenzhen 518116,People’s Republic of China [3]Department of Respiratory,Shenzhen Children’s Hospital,Shenzhen 518038,People’s Republic of China [4]Institute of Pediatrics,Shenzhen Children’s Hospital,Shenzhen 518038,People’s Republic of China [5]Shenzhen Han’s Tech Limited Company,Shenzhen 518000,People’s Republic of China [6]Hospital of Guangzhou Medical University,Qingyuan city People’s Hospital,Qingyuan 511518,People’s Republic of China [7]Department of Physics,Faculty of Science,King Abdulaziz University,Jeddah 21589,Saudi Arabia [8]Research Center for Advanced Materials Science(RCAMS),King Khalid University,P.O.Box 9004,Abha 61413,Saudi Arabia [9]Department of Chemistry,College of Science,King Khalid University,P.O.Box 9004,Abha 61413,Saudi Arabia [10]Zhongmin(Shenzhen)Intelligent Ecology Co.,Ltd,Shenzhen 518055,People’s Republic of China [11]Shenzhen Center for Disease Control and Prevention,Shenzhen 518055,People’s Republic of China

出　　处：《Nano-Micro Letters》2022年第10期83-94,共12页纳微快报（英文版）

基　　金：support from the Innovation Team Project of Department of Education of Guangdong Province(No.2018KCXTD026);Guangdong Scientific and Technological Project(2019B1515120043,2020A151501612,2021A1515220109,2022B1515020093);Science and Technology Innovation Commission of Shenzhen(Grant No.KCXFZ20201221173413038);Longhua District Science and Innovation Commission Project Grants of Shenzhen(JCYJ201904).

摘　　要：Coronavirus disease 2019(COVID-19)is a highly contagious disease caused by severe acute respiratory syndrome coronavirus 2(SARS-CoV-2).The gold standard method for the diagnosis of SARS-CoV-2 depends on quantitative reverse transcription-polymerase chain reaction till now,which is time-consuming and requires expensive instrumentation,and the confirmation of variants relies on further sequencing techniques.Herein,we first proposed a robust technique-methodology of electrochemical CRISPR sensing with the advantages of rapid,highly sensitivity and specificity for the detection of SARS-CoV-2 variant.To enhance the sensing capability,gold electrodes are uniformly decorated with electro-deposited gold nanoparticles.Using DNA template identical to SARS-CoV-2 Delta spike gene sequence as model,our biosensor exhibits excellent analytical detection limit(50 fM)and high linearity(R2=0.987)over six orders of magnitude dynamic range from 100 fM to 10 nM without any nucleic-acid-amplification assays.The detection can be completed within 1 h with high stability and specificity which benefits from the CRISPR-Cas system.Furthermore,based on the wireless micro-electrochemical platform,the proposed biosensor reveals promising application ability in point-of-care testing.

关 键 词：SARS-COV-2 variant Methodology of electrochemical CRISPR sensing(MOECS) Gold nanoparticles(AuNPs) Point-of-care testing(POCT) 

分 类 号：TP212.3[自动化与计算机技术—检测技术与自动化装置] R440[自动化与计算机技术—控制科学与工程]