阿德福韦酯下调HOXB7介导乙型病毒性肝炎相关肝癌细胞的增殖和凋亡  

Adefovir dipivoxil down-regulates HOXB7-mediated proliferation and apoptosis of hepatitis B-related liver cancer cells

作　　者：李可阳 龚丹丹 沈增强 李晓天[1] LI Ke-yang;GONG Dan-dan;SHEN Zeng-qiang;LI Xiao-tian(College of Pharmacy,Zhengzhou University,Zhengzhou 450000,Henan Province,China;Department of Clinical Pharmacy,Jiaozuo People’s Hospital,Jiaozuo 454001,Henan Province,China;Department of Pharmacy,Jiaozuo People’s Hospital,Jiaozuo 454001,Henan Province,China)

机构地区：[1]郑州大学药学院,河南郑州450000 [2]焦作市人民医院临床药学室,河南焦作454001 [3]焦作市人民医院药剂科,河南焦作454001

出　　处：《中国临床药理学杂志》2022年第16期1897-1901,共5页The Chinese Journal of Clinical Pharmacology

摘　　要：目的研究阿德福韦酯(ADV)调控同源盒基因B7(HOXB7)对乙型病毒性肝炎相关肝癌细胞增殖、凋亡影响。方法HepG2.2.15细胞分成对照组、ADV(ADV处理)、si-NC(转染siRNA control)、si-HOXB7(转染HOXB7 siRNA)、ADV+pcDNA-NC(转染pcDNA,给予ADV处理)、ADV+pcDNA-HOXB7组(转染pcDNA-HOXB7,给予ADV处理)。以聚合酶链反应(PCR)法检测乙型病毒性肝炎病毒DNA(HBV-DNA),用细胞计数试剂盒-8(CCK-8)检测细胞增殖,用流式细胞术检测细胞凋亡及HepG2.2.15细胞的周期进程情况,用蛋白质印迹(Western blot)法检测B细胞淋巴瘤-2(Bcl-2)、Bcl-2相关X蛋白(Bax)、胱天蛋白酶3(caspase-3)、HOXB7蛋白表达。结果对照组和ADV组HBV-DNA水平分别为(4.67±0.81)和(3.20±0.67)U·mL^(-1),凋亡率分别为(5.61±0.55)%和(12.73±1.34)%,差异均有统计学意义(均P<0.05)。si-NC组和si-HOXB7组HepG2.2.15细胞凋亡率分别为(5.34±0.58)%和(15.41±1.62)%,差异有统计学意义(P<0.05)。与si-NC组比较,si-HOXB7组细胞中Bax、caspase-3蛋白表达增多,Bcl-2、HOXB7蛋白表达减少(均P<0.05)。与ADV+pcDNA-NC组比较,ADV+pcDNA-HOXB7组HepG2.2.15细胞HBV复制能力增加,增殖能力升高,凋亡率降低,细胞中Bax、caspase-3蛋白表达减少,Bcl-2蛋白表达增多(均P<0.05)。结论阿德福韦酯下调HOXB7抑制乙型病毒性肝炎相关肝癌细胞增殖,促进细胞凋亡。Objective To study the effect of adefovir dipivoxil(ADV)on the proliferation and apoptosis of hepatitis B-related liver cancer cells by regulating homolobox gene B7(HOXB7).Methods HepG2.2.15 cells were divided into control group,ADV(treated with ADV),si-NC(transfected with siRNA control),si-HOXB7(transfected with HOXB7 siRNA),ADV+pcDNA-NC(transfected with pcDNA,given ADV treatment),ADV+pcDNA-HOXB7 group(transfected pcDNA-HOXB7,given ADV treatment).Polymerase chain reaction(PCR)method was used to detect hepatitis B virus-DNA(HBV-DNA);cell counting kit 8(CCK-8)was used to detect cell proliferation;flow cytometry was used to detect cell apoptosis;Western blot was used to detected B-cell lymphoma-2(Bcl-2),Bcl-2-associated X protein(Bax),cysteine protease 3(caspase-3),HOXB7 protein expression.Results The level of HBV-DNA in control group and ADV were(4.67±0.81)and(3.20±0.67)U·mL^(-1);apoptosis rate were(5.61±0.55)% and(12.73±1.34)%;the differences were statistically significant(P<0.05).The apoptosis rates of si-NC group and si-HOXB7 group were(5.34±0.58)% and(15.41±1.62)% and the differences were statistically significant(P<0.05).Compared with si-NC group,the protein expressions of Bax and caspase-3 in si-HOXB7 group were increased,while the protein expressions of Bcl-2 and HOXB7 were decreased(all P<0.05).Compared with ADV+PCDNA-NC group,HBV replication ability,proliferation ability and apoptosis rate of HepG2.2.15 cells in ADV+PCDNA-HOXB7 group were increased;the expression of Bax and caspase-3 protein were decreased;the expression of Bcl-2 protein was increased(all P<0.05).Conclusion Adefovir dipivoxil down-regulates HOXB7 to inhibit the proliferation of hepatitis B-related liver cancer cells and promote cell apoptosis.

关 键 词：乙型病毒性肝炎相关肝癌 阿德福韦酯 同源盒基因B7 凋亡 

分 类 号：R97[医药卫生—药品]