青蒿琥酯联合西妥昔单抗对K-ras突变型腺癌细胞的抗癌活性的影响  

Effect of artesunate combined with cetuximab on anticancer activity of K-ras mutant adenocarcinoma cells

作　　者：王怀涛[1] 高峰[1] 谭晓冬[1] WANG Huai-tao;GAO Feng;TAN Xiao-dong(Department of Pancreatic Thyroid Surgery,Shengjing Hospital,China Medical University,Shenyang 110004,Liaoning Province,China)

机构地区：[1]中国医科大学附属盛京医院胰腺甲状腺外科,辽宁沈阳110004

出　　处：《中国临床药理学杂志》2022年第16期1902-1905,共4页The Chinese Journal of Clinical Pharmacology

摘　　要：目的探究青蒿琥酯对西妥昔单抗对K-ras基因突变胰腺癌细胞抗癌活性的影响。方法体外培养人胰腺癌细胞系(AsPC-1),分为空白组、模型组、对照组和实验组。空白组不进行任何干预,模型组给以10.0μg·mL^(-1)青蒿琥酯,对照组细胞给予100.0μg·mL^(-1)西妥昔单抗,实验组细胞同时给予100.0μg·mL^(-1)西妥昔单抗和10.0μg·mL^(-1)青蒿琥酯进行干预。通过MTT实验、Transwell实验、细胞凋亡实验和克隆形成实验检测青蒿琥酯对西妥昔单抗对胰腺癌细胞的抗癌活性的影响。结果AsPC-1细胞的K-ras基因突变类型为12密码子突变型,突变形式为GAT。模型组、对照组和实验组AsPC-1细胞增殖活力抑制率分别为(15.16±2.87)%,(23.62±4.19)%和(74.68±8.94)%,实验组对AsPC-1细胞的增殖活力抑制率明显升高(P<0.05)。空白组、模型组、对照组和实验组的穿膜细胞数分别为121.67±3.06,96.67±1.53,84.33±3.06和56.33±5.03,细胞凋亡率分别为(8.74±1.13)%,(10.68±2.41)%,(16.74±2.94)%和(25.87±3.19)%,实验组与模型组和对照组比较,差异均有统计学意义(均P<0.05)。模型组、对照组和实验组AsPC-1细胞集落形成的抑制率分别为(8.05±1.54)%,(14.58±1.76)%和(39.16±4.86)%,实验组细胞集落形成抑制率明显高于模型组和对照组(P<0.05)。结论青蒿琥酯可显著增强西妥昔单抗对K-ras基因突变胰腺癌细胞的抗癌活性。Objective To investigate the effect of artesunate on the anti-cancer activity of cetuximab on K-ras mutated pancreatic cancer cells.Methods The in vitro cultured human pancreatic cancer cell line(AsPC-1)were divided into blank group,model group,control group and experimental group.Blank group was not subjected to any intervention;model group was treated with 10.0μg·mL^(-1) artesunate;control group was treated with 100.0μg·mL^(-1) cetuximab;experimental group was treated with 100.0μg·mL^(-1) cetuximab and 10.0μg·mL^(-1) artesunate.The effect of artesunate on the anticancer activity of EGRF inhibitor cetuximab on pancreatic cancer cells was examined by MTT assay,Transwell assay,apoptosis assay and clone formation.Results The mutation type of K-ras gene in AsPC-1 cells was 12 codon mutation and GAT mutation.The inhibition rates of proliferation viability of AsP C-1 cells in model group,control group and experimental group were(15.16±2.87)%,(23.62±4.19)% and(74.68±8.94)% and the inhibition rate of proliferation activity of AsP C-1 cells in experimental group was significantly increased(P<0.05).The number of membrane penetrating cells in blank group,model group,control group and experimental group were 121.67±3.06,96.67±1.53,84.33±3.06 and 56.33±5.03;the apoptosis rates of AsP C-1 cells were(8.74±1.13)%,(10.68±2.41)%,(16.74±2.94)% and(25.87±3.19)%;there were statistically significant differences between experimental group and model group,control group(all P<0.05).The inhibition rates of AsP C-1 cell colony formation in model group,control group and experimental group were(8.05±1.54)%,(14.58±1.76)% and(39.16±4.86)%,and the inhibition rate of cell colony formation in experimental group was significantly higher than those in model group and control group(P<0.05).Conclusion Artesunate significantly enhanced the anticancer activity of cetuximab against K-ras gene mutated pancreatic cancer cells.

关 键 词：青蒿琥酯 表皮生长因子受体抑制药 西妥昔单抗 胰腺癌 K-RAS基因突变 

分 类 号：R28[医药卫生—中药学]