VitD在降低PM_(2.5)对肺泡上皮细胞毒性中的影响分析  

Effect of VitD on reducing the cytotoxicity of PM_(2.5)to alveolar epithelial cells

作　　者：黄婧 王蕾[2] Huang Jing;Wang Lei(Department of Rheumatism and Immunology,First Affiliated Hospital,Xi′an Jiaotong University,Xi′an,710061,China;Department of Pulmonary and Critical Care Medicine,Second Affiliated Hospital,Xi′an Jiaotong University,Xi′an,710004,China)

机构地区：[1]西安交通大学第一附属医院风湿免疫科,西安710061 [2]西安交通大学第二附属医院呼吸与危重症科,西安710004

出　　处：《中华肺部疾病杂志（电子版）》2022年第4期481-485,共5页Chinese Journal of Lung Diseases(Electronic Edition)

基　　金：陕西省自然科学基础研究计划(2022JQ-761);中国博士后科学基金会(2021M702610);中央高校基本科研业务费(xzy012020060)。

摘　　要：目的分析维生素D(vitamin D,VitD)在降低PM_(2.5)对肺泡上皮细胞毒性中的影响。方法采用透射电子显微镜和激光诱导荧光分析对比VitD处理前后,PM_(2.5)的毒性能力。MMT观察VitD处理前后细胞生存率,分析PM_(2.5)对A549的毒性影响。结果VitD作用后的PM_(2.5)平均粒径减小5.5 nm,颗粒聚结和团聚更为明显,颗粒平均条纹长度增加、弯曲度减小,差异有统计学意义。PM_(2.5)条纹间距无统计学差异,有明显减小,接近0.06 nm。VitD溶液从PM_(2.5)解吸3环和4环多环芳香烃(polycyclic aromatic hydrocarbons,PAHs),减少PM_(2.5)表面附着的致病性PAHs。PM_(2.5)可引起肺上皮细胞A549生存率明显下降,给予VitD干预后,PM_(2.5)对A549细胞生存率的抑制改善了54.7%,PM_(2.5)的生物毒性降低。结论VitD可减少PM_(2.5)上吸附的致病性PAHs,抑制其致病活性,减小PM_(2.5)对肺上皮细胞的毒性。Objective To investigate the decreased effect of vitamin D(VitD)on the cytotoxicity of alveolar epithelial cells by PM_(2.5).Methods Comparison of PM_(2.5)toxicity between without and with VitD treatment by transmission electron microscopy and laser induced fluorescence.MTT observed the effect of PM_(2.5)on the survival rate of A549 cells before and after VitD treatment.Results After the treatment of VitD,the average diameter of PM_(2.5)was decreased 5.5 nm.The particle agglomeration and coalescence were more obvious.The average fringe length increased and fringe tortuosity decreased,the statistics difference was significant.There was no statistical difference in the fringe spacing of particle,but there was a significant reduction,which was close to 0.06 nm.VitD solution desorbed 3-ring and 4-ring PAHs from PM_(2.5),reducing pathogenic PAHs on PM_(2.5).PM_(2.5)can cause a significant lung epithelial cells A549 cell death,and this effect is significantly reversed by 54.7%after VitD intervention,indicating that the biotoxicity of PM_(2.5)is reduced by VitD.Conclusion VitD can strip the pathogenic PAHs from PM_(2.5).Then it can significantly inhibit the pathogenic activity of PM_(2.5)and reduce the cytotoxicity of PM_(2.5).

关 键 词：PM_(2.5) 维生素D 多环芳香烃 生物毒性 

分 类 号：R392.12[医药卫生—免疫学]