β-细辛醚对帕金森病抑郁大鼠前额叶皮质自噬通路及突触可塑性的影响  

Effects of β-Asarone on Autophagy Signaling and Synaptic Plasticity in the Prefrontal Cortex of the Rats with Parkinson’s Disease Depression

作　　者：王志芳 宁百乐 康健 方永奇[3] 李翎[3] WANG Zhi-fang;NING Bai-le;KANG Jian;FANG Yong-qi;LI Ling(Guangzhou University of Chinese Medicine,Guangzhou Guangdong,510006,China;Second Affiliated Hospital of Guangzhou University of Chinese Medicine,Guangzhou Guangdong,510120 China;First Affiliated Hospital of Guangzhou University of Chinese Medicine,Guangzhou Guangdong,510006,China)

机构地区：[1]广州中医药大学,广东广州510006 [2]广州中医药大学第二附属医院,广东广州510120 [3]广州中医药大学第一附属医院,广东广州510006

出　　处：《时珍国医国药》2022年第7期1560-1563,共4页Lishizhen Medicine and Materia Medica Research

基　　金：国家自然科学基金青年基金(81804166);广东省广州市科技计划项目(202102010247);广东省科技计划项目(2016A030303053);广东省中医药局中医药科研项目(20211146)。

摘　　要：目的 探讨β-细辛醚对帕金森病抑郁(Depression in Parkinson’s disease,DPD)大鼠前额叶皮质自噬水平及突触可塑性的影响。方法 用6-OHDA诱导结合CUMS方法建立DPD模型大鼠并随机分组,同时设假手术组。比较假手术组、模型组、普拉克索组、帕罗西汀组、β-细辛醚低、中、高剂量组的行为学表现(OFT、PST、SPT)和纹状体α-syn的含量变化;比较假手术组、模型组、β-细辛醚中剂量组、3-MA组和雷帕霉素组大鼠前额叶皮质Beclin-1、p62和LC3I/II蛋白的表达水平和突触相关蛋白p-p70s6k、p70s6k、PSD95和synapsin 1的表达水平,观察各组大鼠神经元的形态结构。结果 β-细辛醚能改善DPD模型大鼠的运动状态、提高糖水偏好率和降低强迫游泳不动时间,减少纹状体ɑ-syn的含量,下调大鼠前额叶皮质Beclin-1和LC3I/II的表达,上调p62的表达,促进突触相关蛋白PSD95、synapsin 1、p-p70s6k和p70s6k的表达,β-细辛醚组大鼠的神经元形态结构与3-MA组相似,神经元损伤程度较轻。结论 β-细辛醚可通过抑制大鼠前额叶皮质自噬水平、调控突触可塑性,保护神经元结构,从而改善DPD模型大鼠的抑郁症状。Objective Discuss the effects of β-asarone on the prefrontal cortex autophagy level and synaptic plasticity of the rats with depression in Parkimson’s disease(DPD).Methods 6-OHDA induction and CUMS method were used to establish the DPD model rats,the rats were subject to random grouping,the sham-operated group was set,and the changes in behavioral performance(OFT,PST and SPT) and α-syn levels in corpus striatum of sham-operated group,model group,pramipexole group,paroxetine group,group with low β-asarone,group with medium β-asarone and group with high β-asarone were compared;The expression levels of Beclin-1,p62 and LC3 I/II proteins in the prefrontal cortex of rats and the expression levels of synapse-related protein p-p70 s6 k,p70 s6 k,PSD95 and synapsin 1 of sham-operated group,model group,β-asarone medium dose group,3-MA group and rapamycin group were compared and the nerve cell morphology of the rats in all groups were observed.Results The β-asarone can increase the movement distance of DPD model rats,enhance the sugar water preference rate and decrease the forced swimming immobility time,reduce the content of ɑ-syn in the corpus striatum,down-regulate the Beclin-1 and LC3 I/II expression in the prefrontal cortex of rats,up-regulate the p62 expression,and promote the expression of synapse-related protein PSD95,synapsin 1,p-p70 s6 k and p70 s6 k with the nerve cell morphology of rats in the β-asarone group being similar to that of 3-MA group,which reduced the degree of neuronal injury.Conclusion The β-asarone can regulate the synaptic plasticity by inhibiting the level of autophagy in the prefrontal cortex of rats to improve the depressive symptoms of DPD model rats.

关 键 词：Β-细辛醚 帕金森病抑郁 自噬 突触可塑性 

分 类 号：R285.5[医药卫生—中药学]