灵芝子实体标志性成分与生物活性的研究  

Study on marker components and biological activity of Ganoderma lucidum fruiting bodies

作　　者：林冬梅[1] 罗虹建[1] 马昂 王赛贞[1] 林树钱[1] 杨宝学[2] 林占熺[1] 王联福 LIN Dong-mei;LUO Hong-jian;MA Ang;WANG Sai-zhen;LIN Shu-qian;YANG Bao-xue;LIN Zhan-xi;WANG Lian-fu(National Engineering Research Center of JUNCAO Technology Fujian Agriculture and Forestry University,Fuzhou 350002,China;Department of Pharmacology,School of Basic Medical Sciences,Peking University,Beijing 100038,China)

机构地区：[1]福建农林大学国家菌草工程技术研究中心,福建福州350002 [2]北京大学基础医学院药理学系,北京100038

出　　处：《时珍国医国药》2022年第7期1568-1571,共4页Lishizhen Medicine and Materia Medica Research

基　　金：中央引导地方科技发展专项(2021L3008);福建农林大学学科交叉融合推动菌草科学及产业高质量发展项目(XKJC 71202103A);福建省科技重大专项(2021NZ029009)。

摘　　要：目的 研究灵芝子实体标志性成分——多糖肽和灵芝酸,旨在研究制备活性物质工艺,建立专属性强的分析方法,为提升灵芝质量标准提供参考。方法 采用中空纤维膜分离技术,截留不同分子量组分,再通过色谱法分离纯化得到标志性成分并验证其药理活性。结果 灵芝水提液通过膜分离技术和制备液相可同时得到灵芝多糖肽组分和灵芝酸单体,并作为衡量灵芝子实体质量的标准物质。药理实验表明,灵芝多糖肽具有免疫激活作用和灵芝酸具有抗炎活性。结论 用灵芝活性组分多糖肽和灵芝酸标识灵芝质量,为育种栽培、灵芝提取物生产工艺及产品质量控制提供理论依据。Objective To study the marker components polysaccharide peptide(PP) and ganoderic acid(GA) of Ganoderma lucidum(G.lucidum) fruiting body,and their preparation process,and to establish a specific analytical method,which provides reference for improving the quality standard of G.lucidum.Methods Hollow fiber membrane separation technology was used to abtain different molecular weight components,and then separated and purified by chromatography to obtain PP and GA,as well as verified their pharmacological activities.Results G.lucidum water extract could simultaneously obtain PP component and GA monomer through membrane separation technology and liquid phase preparation,which can be used as standard materials for measuring the quality of G.lucidum fruiting bodies.Pharmacological experiments showed that PP had immune activation effect and GA had anti-inflammatory activity.Conclusion G.lucidum active components PP and GA are used to identify the quality of G.lucidum,which provides the theoretical basis for breeding cultivation,extract production technology and product quality control.

关 键 词：灵芝子实体 标志性成分 生物活性 质量控制 多糖肽 灵芝酸 

分 类 号：R284.1[医药卫生—中药学]