枸杞自交不亲和基因型(S基因)研究进展  

Research Progress on Self-Incompatibility Genotype(S gene)of Lycium barbarum L.

作　　者：高燕 秦垦 焦恩宁 张银霞[1] Gao Yan;Qin Ken;Jiao Enning;Zhang Yinxia(School of Agriculture,Ningxia University,Yinchuan,Ningxia 750021;Institute of Wolfberry Sciences,Ningxia Academy of Agriculture and Forestry Sciences,Yinchuan,Ningxia 750002)

机构地区：[1]宁夏大学农学院,宁夏银川750021 [2]宁夏农林科学院枸杞科学研究所,宁夏银川750002

出　　处：《宁夏农林科技》2022年第3期5-12,共8页Journal of Ningxia Agriculture and Forestry Science and Technology

基　　金：宁夏回族自治区农业育种专项(2018NYYZ0102);宁夏自然科学基金项目(2020AAC03290)。

摘　　要：枸杞是典型的配子体型自交不亲和经果林树种,生产上授粉树的合理选配及杂交亲本的优良组合均受限于自交亲和指数及S-RNase基因。本文系统介绍了运用杂交授粉试验、蛋白质产物分析、cDNA克隆技术、DNA序列分析和靶向测序基因型分型技术等确定枸杞自交不亲和基因型的研究进展,总结运用不同技术研究枸杞自交不亲和性的成果并对比分析各技术的优点和不足,以期为枸杞遗传育种研究提供有价值的参考,为生产上合理选配授粉树提供基础理论依据。Lycium barbarum L.is a typical gametophytic self-incompatibility fruit forest.The reasonable selection of pollination trees and the excellent combination of hybrid parents are limited by the self-compatibility index and S-RNase gene.In this paper,the technical progress of determining the self-incompatibility genotypes of Lycium barbarum L.by hybridzation pollination test,protein product analysis,cDNA cloning technology,DNA sequence analysis and targeted sequencing genotyping technology were systematically introduced.The advantages and disadvantages of each technique in determining self-incompatibility genotypes of Lycium barbarum L.were compared and analyzed.The results of self-incompatibility study of Lycium barbarum L.by using different techniques were summarized,in order to provide a theoretical basis for the rational selection of pollination trees in production,and provide a valuable reference for the genetic and breeding research of Lycium barbarum L..

关 键 词：枸杞 自交不亲和 S-RNASE基因 鉴定技术 

分 类 号：S567.19[农业科学—中草药栽培]