碱蓬根际和内生细菌菌株对盐碱胁迫下苜蓿生长的影响  

Effects of Suaeda glauca rhizobacteria and endophytic bacterial strains on alfalfa growth under salt-alkaline stress

作　　者：苗阳阳 张艳蕊 宋标 刘旭桐 张安琪 吕金泽 张浩 张小华 欧阳佳慧 李旺 曲善民 MIAO Yang-yang;ZHANG Yan-rui;SONG Biao;LIU Xu-tong;ZHANG An-qi;LV Jin-ze;ZHANG Hao;ZHANG Xiao-hua;OUYANG Jia-hui;LI Wang;QU Shan-min(Department of Grassland Science,College of Animal Science and Veterinary Medicine,Heilongjiang Bayi Agricultural University,Key Laboratory of Efficient Utilization and Nutrition Regulation of Feed Resources in Cold Regions of Heilongjiang Province,Daqing 163319,China)

机构地区：[1]黑龙江八一农垦大学动物科技学院草业科学系,黑龙江省寒区饲料资源高效利用与营养调控重点实验室,黑龙江大庆163319

出　　处：《草业学报》2022年第9期107-117,共11页Acta Prataculturae Sinica

基　　金：黑龙江八一农垦大学学成、引进人才科研启动计划项目(XYB201813);黑龙江八一农垦大学三横三纵支持计划项目(ZRCPY201908);大庆市新能源领域“揭榜挂帅”科技攻关项目(2021BD05);黑龙江省科技厅省院合作项目(YS16B12)资助。

摘　　要：为研究碱蓬根际和内生细菌菌株对盐碱胁迫下苜蓿幼苗生长的影响,试验以龙牧801紫花苜蓿为供试品种,将前期从碱蓬根内(JG1)、根际土壤(JT4)和茎内(JJ5)筛选出的具有较强耐盐碱胁迫能力的根际及内生细菌菌株接种于苜蓿幼苗根部。接菌1周后将中性盐(NaCl、Na_(2)SO_(4))和碱性盐(NaHCO_(3)、Na_(2)CO_(3))按NaCl∶Na_(2)SO_(4)∶NaHCO_(3)∶Na_(2)CO_(3)=9∶1∶1∶9混合,并设置0、100、150、200 mmol·L^(-1)盐碱浓度溶液浇灌苜蓿幼苗根部,进行盐碱胁迫处理。分析盐碱胁迫下碱蓬根际及内生细菌菌株对苜蓿生长及生理的影响,并通过隶属函数和主成分分析综合评价碱蓬根际和内生细菌菌株对盐碱胁迫下苜蓿生长的影响。结果表明:3株促生细菌均能缓解盐碱胁迫对紫花苜蓿生长的抑制作用,促进紫花苜蓿生长,不同促生细菌对盐碱胁迫下苜蓿促生效果为JT4>JG1>JJ5。This research investigated the effects of rhizobacteria and endophytic bacteria strains on alfalfa seedling growth under salt-alkaline stress in alfalfa(Medicago sativa)cultivar Longmu No.801.Rhizobacteria and endophytic bacteria from plants with strong tolerance to salt-alkaline stress were isolated from Suaeda glauca roots(JG1),rhizosphere soil(JT4)and stems(JJ5)and were inoculated around the roots of alfalfa seedlings.One week after inoculation,neutral salts(NaCl,Na_(2)SO_(4))and alkaline salts(NaHCO_(3),Na_(2)CO_(3))were mixed in the proportions NaCl∶Na_(2)SO_(4)∶NaHCO_(3)∶Na_(2)CO_(3)=9∶1∶1∶9,and the roots of alfalfa seedlings were irrigated with 0,100,150,200 mmol·L^(-1) of these salt-alkaline solutions.The effects on the growth and physiological indexes of alfalfa of these inoculations under the four salt concentrations were analyzed by membership function and principal component analysis.It was found that the three growth-promoting bacteria alleviated the inhibitory effects of salt-alkaline stress on alfalfa and promoted alfalfa growth.The growth-promoting effect of the isolated bacterial strains on alfalfa seedlings under salt-alkaline stress was JT4>JG1>JJ5.

关 键 词：碱蓬 根际细菌 内生细菌 盐碱胁迫 苜蓿 

分 类 号：S541.9[农业科学—作物学]