基于四面体DNA纳米材料的DNA传感器的研制  

Development of DNA Sensor Based onTetrahedral DNA Nanomaterials

作　　者：刘嘉丽 邓燕 李秋霞[1] 管燕 杨通 孟爽 杨云慧[1] 胡蓉[1] LIU Jia-Li;DENG Yan;LI Qiu-Xia;GUAN Yan;YANG Tong;MENG Shuang;YANG Yun-Hui;HU RONG(Faculty of Chemistry and Chemical Engineering,Yunnan Normal University,Kunming 650500,China)

机构地区：[1]云南师范大学化学化工学院,昆明650500

出　　处：《分析化学》2022年第9期1308-1318,共11页Chinese Journal of Analytical Chemistry

基　　金：国家自然科学基金项目(No.21765026)资助。

摘　　要：四面体DNA纳米材料(TDN)具有机械性能优越、结构稳定、合成方法简单、合成产率高、可特异性识别等优点。本研究构建了一种基于TDN的电化学传感平台用于高灵敏检测DNA。使用性能优良的石墨烯-纳米金颗粒复合材料作为基底,可以进一步提高灵敏度。利用金属有机框架材料Fe-MIL-88-NH_(2)(Fe-MOF)作为信号探针,检测时不需要酸处理与预处理过程,可以节约检测时间。同时,Fe-MOF易功能化,表面负载贵金属纳米粒子后,易修饰上核酸或蛋白质分子,简化了修饰过程。当目标DNA存在时,通过检测Fe-MOF材料的信号峰,实现了对DNA的检测。本方法检测目标DNA的线性范围为0.01~140 pmol/L,线性方程为I=2.858×lg c(pmol/L)+6.488(R^(2)=0.9935),检出限(LOD,3σ)为4.5 fmol/L。Tetrahedral DNA nanomaterial(TDN)is a simple and strong pyramidal three-dimensional structure model.It has the advantages such as superior mechanical properties,stable structure,simple synthesis method,high synthesis yield,and unique specific recognition performance.In this work,a TDN-based electrochemical sensing platform was constructed for highly sensitive detection of DNA.By using Fe-metal organic framework(Fe-MOF)as signal probe,the detection did not require acid treatment and pretreatment process,which could save detection time.In addition,Fe-MOF was easy to functionalize.After the surface was loaded with precious metal nanoparticles,it was easy to modify nucleic acid or protein molecules,which could simplify the modification process.By using graphene-nano-gold particle composite materials with excellent performance as the substrate,it could further improve the sensitivity.When the target DNA was present,the purpose of detecting DNA was achieved by detecting the signal peak in the Fe-MOF material.In the concentration range of 0.01-140 pmol/L,the linear equation was I=2.858×lg c(pmol/L)+6.488(R^(2)=0.9935),and the detection limit(3σ)was 4.5 fmol/L.

关 键 词：四面体DNA纳米材料 Fe-金属有机框架材料 DNA传感器 

分 类 号：Q523[生物学—生物化学] O657.1[理学—分析化学] TP212.3[理学—化学]