氢键促进对乙酰氨基酚在模拟细胞膜表面的富集  

Accumulation of Paracetamol on Surface of SimulatedCell Membrane Promoted by Hydrogen Bonding

作　　者：李霞 陈壮壮 范爱华 张丽丽 邵会波 LI Xia;CHEN Zhuang-Zhuang;FAN Ai-Hua;ZHANG Li-Li;SHAO Hui-Bo(School of Chemistry and Chemical Engineering,Beijing Institute of Technology,Beijing 102488,China)

机构地区：[1]北京理工大学化学与化工学院,北京102488

出　　处：《分析化学》2022年第9期1364-1372,共9页Chinese Journal of Analytical Chemistry

基　　金：国家自然科学基金项目(No.21872006)资助。

摘　　要：广谱解热镇痛药物扑热息痛(对乙酰氨基酚,APAP)在细胞膜表面借助于氢键促进的富集对其发挥药效作用非常重要。本研究以表面含有氢受体(荷负电的羧酸根)的3-巯基丙酸自组装膜(MPA SAM)作为模拟细胞膜表面的简化模型,通过扫描电化学显微镜(SECM)方法研究了氢键促进的APAP在模拟细胞膜表面的富集作用。实验结果表明,随着Mg^(2+)浓度的增大或pH值的升高,MPA SAM表面羧基解离平衡正向移动,产生了更多的羧酸根(氢受体),促进了APAP在MPA SAM表面的富集。采用SECM成像模式实现了上述富集过程的可视化考察。本研究对理解APAP在生物膜表面借助于氢键的富集过程以及APAP传感器的设计提供了参考。The widely used paracetamol(acetaminophen,APAP)for antipyretic and analgesic promoting enrichment on the cell membrane surface by means of hydrogen bonds is very important.In this work,3-mercaptopropionic acid self-assembled monolayer(MPA SAM)containing hydrogen acceptors(negatively charged carboxylates)on the surface was used as simplified model to simulate the cell membrane surface.The enrichment of APAP promoted by hydrogen bonds on the surface of simulated cell membranes was studied by scanning electrochemical microscopy(SECM).The experimental results showed that with the increase of Mg^(2+) concentration or pH value,the carboxyl groups of MPA SAM dissociated and shifted positively to produce more carboxylates(hydrogen acceptors).The enrichment of APAP on the surface of MPA SAM was promoted.Furthermore,SECM image mode realized the visualization of enrichment.This work might provide a certain reference for understanding the enrichment of APAP promoted by hydrogen bonds on the surface of simulated cell membrane and the design of APAP sensors.

关 键 词：对乙酰氨基酚 氢键 富集 自组装单分子膜 扫描电化学显微镜 

分 类 号：R965[医药卫生—药理学]