营养生长期持续高温处理对玉米叶片转录组及生化指标的影响  

Effects of Continuous High Temperature Treatment during Vegetative Stages on Maize Leaf Transcriptome and Biochemical Indicators

作　　者：陈岩 岳丽杰[1] 刘永红[1] 杨勤[1] CHEN Yan;YUE Li-jie;LIU Yong-hong;YANG Qin(Crop Research Institute,Sichuan Academy of Agricultural Sciences,Chengdu 610066,China)

机构地区：[1]四川省农业科学院作物研究所,成都610066

出　　处：《玉米科学》2022年第4期48-55,61,共9页Journal of Maize Sciences

基　　金：国家重点研发计划项目(2017YFD0300304);国家玉米产业技术体系项目(CARS-02-19)。

摘　　要：以玉米杂交种郑单958和登海605为材料,通过转录组测序分析玉米营养生长期叶片受热胁迫后基因差异表达、基因功能富集和KEGG通路富集,使用微量法和HPLC法分析叶片中相关生化指标的变化。结果表明,高温胁迫会降低光合作用关键酶二磷酸核酮糖羧化酶(Rubisco)和三羧酸循环限速酶柠檬酸合酶(CS)的活力,升高丙二醛(MDA)、脱落酸(ABA)和赤霉素(GA3)的含量,降低水杨酸(SA)的含量。与常温对照比,玉米营养生长期叶片受热胁迫后,两个品种4个比较组合累计检测到16996个差异表达的基因,共同差异表达的基因有1160个。光合作用、细胞代谢、叶绿体膜和质体膜等相关基因的表达会响应热胁迫。The maize hybrids Zhengdan958 and Denghai605 were used as experimental materials,the gene differential expression,gene function enrichment and KEGG pathway enrichment in maize leaves after heat treatment were analyzed by transcriptome sequencing.The changes of related biochemical substances in leaves were analyzed by microscopic and HPLC methods.The results showed that heat stress could reduce the key enzyme activities of photosynthesis and cell metabolism,such as Rubisco and CS,it could increase the content of MDA,ABA,and GA3in maize leaves,and reduce the content of SA.Compared with the control groups,the expression of many genes was up or down-regulated after continuous heat treatment.16996 differentially expressed genes(DEGs)were detected in four comparison combinations of two varieties,among which 1160 DEGs were co-detected.Functional and pathway enrichment analysis showed that the expression of genes would respond to heat stress,such as genes related to photosynthesis,cell metabolism,chloroplast membrane and plastid membrane.

关 键 词：玉米 高温胁迫 转录组 差异表达基因 富集分析 表型 

分 类 号：S513.01[农业科学—作物学]