补肾活血方对去卵巢大鼠骨质疏松症模型大鼠OPG/RANK/RANKL信号通路的影响  

Effect of Bushen Huoxue formula on OPG/RANK/RANKL signaling pathway in ovariectomized model rats with osteoporosis

作　　者：杨晓江 刘凯[1] 孙绍裘 孙绍卫 YANG Xiao-jiang;LIU Kai;SUN Shao-qiu(Hunan University of Traditional Chinese Medicine,Changsha 410208,China;不详)

机构地区：[1]湖南中医药大学,长沙410208 [2]湖南中医院大学第二附属医院

出　　处：《山西中医》2022年第9期59-61,66,共4页Shanxi Journal of Traditional Chinese Medicine

基　　金：湖南省教育厅科学研究项目(编号:17C1230)。

摘　　要：目的:通过检测血清OPG、RANK、RANKL水平及骨密度变化情况,观察补肾活血方对去卵巢骨质疏松模型大鼠OPG/RANK/RANKL信号通路的影响,以探讨其防治绝经后骨质疏松症的作用机制。方法:选取3月龄的雌性SD大鼠32只,随机分成中药组、雌激素组、模型组、空白对照组各8只,适应性喂养1周后,对中药组、雌激素组、模型组摘除双侧卵巢造模,空白对照组切除卵巢周围等体积大小的脂肪。2个月后中药组予补肾活血方灌胃,雌激素组予结合雌激素片溶液灌胃,模型组及空白对照组均予等体积0.9%生理盐水灌胃,每日1次。连续灌胃3个月后取材,通过ELISA法检测血清OPG、RANK、RANKL水平,并检测大鼠骨密度情况,结果进行统计分析。结果:空白对照组骨密度值高于其余各组(P﹤0.01),且模型组骨密度值较中药组、雌激素组更低(P﹤0.01)。空白对照组、中药组及雌激素组OPG水平均较模型组高(P﹤0.01),且中药组及雌激素组OPG水平均较空白对照组高(P﹤0.01)。空白对照组、中药组及雌激素组RANK水平均较模型组低(P﹤0.01)。空白对照组、中药组及雌激素组的RANKL水平均较模型组低(P﹤0.01),中药组及雌激素组RANKL水平均低于空白对照组(P﹤0.01)。结论:补肾活血方可通过促进OPG的表达,抑制RANK、RANKL的表达,进而促进骨形成,抑制骨吸收,达到有效防治绝经后骨质疏松症的目的。Objective:By detecting the changes of serum OPG, RANK, RANKL levels and bone mineral density,to observe the effect of Bushen Huoxue formula on OPG/RANK/RANKL signaling pathway in ovariectomized model rats with osteoporosis, so as to approach the mechanism of prevention and treatment of postmenopausal osteoporosis.Methods:32 female SD rats of 3-month-old were selected and randomly divided into four groups: traditional Chinese medicine(TCM)group, estrogen group, model group and blank control group, 8 rats in each group. After adaptive feeding for one week, TCM group, estrogen group and model group were removed the bilateral ovaries and modeled.Blank control group was removed the fat of same volume around ovaries. After 2 months, TCM group was given Bushen Huoxue formula by gavage, estrogen group was given conjugated estrogen tablets solution by gavage, model group and blank control group were given equal volume of 0.9% normal saline by gavage, once a day. After 3 months of continuous intragastric administration, samples were taken. The levels of serum OPG, RANK and RANKL were detected by ELISA. The bone mineral density of rats was detected. The results were statistically analyzed. Results:The bone mineral density of rats in blank control group was higher than that of rats in TCM group, estrogen group and model group(P﹤0.01);the bone mineral density of model group was lower than that of TCM group and estrogen group(P ﹤0.01).The OPG levels of rats in blank control group,TCM group and estrogen group were higher than that in model group(P﹤0.01);the OPG levels of rats in TCM group and estrogen group were higher than that in blank control group(P﹤0.01).RANK levels of rats in blank control group,TCM group and estrogen group were lower than that in model group(P﹤0.01).RANKL levels in blank control group,TCM group and estrogen group were lower than that in model group(P﹤0.01);RANKL levels of rats in TCM group and estrogen group were lower than that in blank control group(P﹤0.01).Conclusion:Bushen Huoxue formul

关 键 词：绝经后骨质疏松症 补肾活血方 OPG/RANK/RANKL信号通路 实验研究 

分 类 号：R681[医药卫生—骨科学]