烟草黑胫病菌胶体金免疫层析快速检测技术的建立与评价  

Development and Evaluation of Colloidal Gold Immunochromatography Technique for Rapid Detection of Phytophthora nicotianae

作　　者：杨洋[1] 羊国根 杨懿德 许大凤[3] 崔权仁[3] 李林秋 鄢敏 周本国[3] 潘月敏[2] YANG Yang;YANG Guo-gen;YANG Yi-de(Yibin Company of Sichuan Tobacco Company,Yibin,Sichuan 644600;School of Plant Protection,Anhui Agricultural University,Hefei,Anhui 230036)

机构地区：[1]四川省烟草公司宜宾市公司,四川宜宾644600 [2]安徽农业大学植物保护学院,安徽合肥230036 [3]安徽省农业科学院烟草研究所,安徽合肥230031

出　　处：《安徽农业科学》2022年第17期128-131,137,共5页Journal of Anhui Agricultural Sciences

基　　金：四川省烟草公司科技项目“烟草黑胫和根黑腐病原菌快检方法研究与产品开发”(SCYC201904)。

摘　　要：以烟草黑胫病为研究对象旨在开发一种快速、简单、灵敏的胶体金免疫层析快速检测技术。制备了一种抗烟草黑胫病菌单克隆抗体,并将其应用于胶体金免疫层析试纸研制;在5~10 min就可以检测到病原菌,检测极限为0.1μg/mL抗原。烟草黑胫病菌胶体金免疫层析快速检测技术与常规PCR技术相比,胶体金速测卡检出率略低于常规PCR检测。The purpose of this study was to develop a rapid,simple and sensitive colloidal gold immunochromatographic technique for the rapid detection of tobacco black shank.In this study,a monoclonal antibody against tobacco black shank pathogen was prepared and applied to immunochromatographic test paper;pathogen can be detected within 5-10 minutes,and the detection limit was 0.1μg/mL antigen.Compared with PCR,the detection rate of colloidal gold rapid detection card was slightly lower than PCR.

关 键 词：烟草黑胫病菌 胶体金免疫层析 快速检测 

分 类 号：S435.72[农业科学—农业昆虫与害虫防治]