煤层原位微生物群落结构和功能预测研究  

Microbial community structure and function prediction of in-situ coal samples

作　　者：牛显 牛煜 索永录[1] NIU Xian;NIU Yu;SUO Yong-lu(College of Energy Science and Engineering,Xi’an University of Science and Technology,Xi’an 710054,China;School of Mining and Technology,Inner Mongolia University of Technology,Hohhot 010051,China;School of Electric Power,Civil Engineering and Architecture,Shanxi university,Taiyuan 030006,China)

机构地区：[1]西安科技大学能源学院,陕西西安710054 [2]内蒙古工业大学矿业学院,内蒙古呼和浩特010051 [3]山西大学电力与建筑学院,山西太原030006

出　　处：《煤炭工程》2022年第8期149-156,共8页Coal Engineering

基　　金：内蒙古自治区自然科学基金资助项目(2020MS05025);内蒙古工业大学科学研究项目资助项目(ZY201823);山西省基础研究计划(自由探索类)(202103021223031)。

摘　　要：掌握原位煤层中本源微生物群落结构与功能是探索和控制原位煤层生物转化的有效途径。为准确地反应原位菌群,利用地质钻孔沿垂直方向采集不同埋深新揭露煤层作为煤样,并通过16S rRNA和18S rRNA高通量测序对原位煤样的细菌、古细菌和真菌群落的Alpha多样性、群落组成与差异进行了分析。最后,基于KEGG和MetaCyc数据库进行了二级功能预测。在原位煤样中,微生物群落丰富度随煤样埋深增加而降低。其中,细菌共鉴定出27门67纲159目267科468属,以变形菌门和水杆菌属为优势门和属。古细菌共鉴定出6门14纲21目26科31属,以广古细菌门、Asgardaeota门和泉古细菌门为优势门,且优势属中发现有甲烷杆菌属。真菌群落共鉴定出20门38纲52目66科72属,以链形植物门、子囊菌门和Colpodellidae门为优势门,优势属为玉蜀黍属、Colpodella属和复膜孢酵母属。功能预测表明细菌、古细菌和真菌主要以维持基本生命活动的物质和能量进行代谢。研究结果将为煤层气生物工程区块优选提供参考。Mastering the indigenous microbial community structure and function in in-situ coal seams is an effective way to explore and control the biotransformation of in-situ coal seams.In order to accurately reflect the in-situ flora,newly exposed coal samples at different burial depths were collected using geological drilling.Through 16 S rRNA and 18 S rRNA high-throughput sequencing,the Alpha diversity,community composition and differences of the bacterial,archaeal and fungal communities in in-situ coal samples were analyzed.Finally,secondary function prediction was performed based on KEGG and MetaCyc databases.In the in-situ coal samples,the microbial community richness decreased with increasing of coal sample burial depth.Among them,bacteria identified 27 phyla,67 classes,159 orders,267 families and 468 genera,with Proteobacteria and Aquabacteriumas the dominant phyla and genera.Archaea identified 6 phyla,14 classes,21 orders,26 families and 31 genera.The dominant phyla of archaea were Euryarchaeota,Asgardaeota and Crenarchaeota,and methanobacterium was found among the dominant genera.The fungal community obtained 20 phyla,38 classes,52 orders,66 families and 72 genera;the dominant phyla of fungi included the Streptophyta,Ascomycota and Colpodellidae,and the dominant genera were Zea,Colpodella and Saccharomycopsis.The main functional prediction of microbial communities was the metabolism of substances and energy required to maintain basic microbial life activities.The research results will provide a reference for the preferential selection of coalbed methane bioengineering blocks.

关 键 词：原位煤样 高通量测序 群落组成 物种多样性 功能预测 

分 类 号：X703[环境科学与工程—环境工程]