全自动酶免仪与免疫发光仪检测乙肝两对半定量的对比研究  

A Comparative Study on the Two-pair Semi-quantitative Detection of Hepatitis B by Automatic Enzyme Immunoassay Instrument and Immunoluminescence Instrument

作　　者：赵鼎铭 廖久棋 ZHAO Dingming;LIAO Jiuqi(Huludao Central Blood Station,Huludao 125000,China;Huludao Central Hospital,Huludao 125000,China)

机构地区：[1]葫芦岛市中心血站,辽宁葫芦岛125000 [2]葫芦岛市中心医院,辽宁葫芦岛125000

出　　处：《中国医药指南》2022年第24期113-115,共3页Guide of China Medicine

摘　　要：目的 探讨在乙肝两对半定量研究中分别应用全自动酶免仪与免疫发光仪检测的临床价值。方法 选取我院于2018年12月至2019年12月进行门诊就诊及住院治疗的患者100例,所有患者均同时进行全自动酶免仪与免疫发光仪检测,其中对照组的结果为患者行免疫发光仪检测的结果,研究组的结果为患者行全自动酶免仪检测的结果,对比乙肝两对半定量检测中有1项以上呈阳性的概率及全为阴性的概率。结果 对照组检出1项以上呈阳性的概率有79例,全为阴性概率为21例;而研究组阳性概率有80例,全为阴性概率有20例,组间差异无统计学意义(P> 0.05)。经阳性测试后发现,研究组、对照组抗-HBc(+)分别为5例(5.00%)、11例(11.00%);抗-HBs(+)分别为26例(26.00%)、21例(21.00%);抗-HBc(+)、抗-HBe(+)分别为4例(4.00%)、6例(6.00%);抗-HBc(+)、抗-HBe(+)、抗-HBs(+)分别为18例(18.00%)、14例(14.00%);抗-HBc(+)、抗-HBs(+)分别为10例(10.00%)、4例(4.00%);抗-HBe(+)、抗-HBs(+)分别为2例(2.00%)、5例(5.00%);抗-HBs Ag(+)、抗-HBc(+)分别为2例(2.00%)、1例(1.00%);抗-HBs Ag(+)、抗-HBc(+)、抗-HBe Ag(+)分别为5例(5.00%)、10例(10.00%);抗-HBs Ag(+)、抗-HBc(+)、抗-HBe(+)分别为7例(7.00%)、5例(5.00%);抗-HBs Ag(+)、抗-HBs(+)分别为1例(1.00%)、2例(2.00%);乙肝两对半1项以上呈阳性的概率组间差异无统计学意义,P> 0.05。结论 两种乙肝检测方式均具有较高的准确性,但全自动酶免仪的灵敏性更高,且具有较快的检测速度,在检测过程中能有效规避交叉污染,具有高效、稳定的特点。Objective To investigate the clinical value of automatic enzyme immunoassay and immunoluminescence assay in the two-half quantitative study of hepatitis b.Methods Elected to the hospital December 2018 to December 2019 with outpatient and hospitalization of patients during the 100 cases,all patients were simultaneously automatic enzyme immunoassay instrument with immune luminescence instrument detection.Among them,the results of the control group are the results of the patients undergoing immunoluminescence detection,and the results of the research group are the results of the patients undergoing automatic enzyme immunoassay detection.Compared with the two pairs of semi-quantitative detection of hepatitis B,the probability of positive for more than one item and the total of probability of being negative.Results In the control group,the probability of more than one item being positive was 79 cases,and the probability of all items being negative was 21 cases.In the study group,80 cases were positive and 20 cases were all negative,and there was no significant difference between the two groups(P>0.05).After positive test,anti-HBc(+)was found in 5 cases(5.00%)in the study group and 11 cases(11.00%)in the control group.Anti-HBs(+)was 26 cases(26.00%the study gruoup)and 21 cases(21.00%the control gruop),respectively.Anti-HBc(+)and anti-HBe(+)were 4 cases(4.00%the study gruoup)and 6 cases(6.00%the control gruop),respectively.Anti-HBc(+),anti-HBe(+)and anti-HBs(+)were 18 cases(18.00%the study gruoup)and 14 cases(14.00%the control gruop),respectively.Anti-HBc(+)and anti-HBs(+)were 10 cases(10.00%the study gruoup)and 4 cases(4.00%the control gruop),respectively.Anti-HBe(+)and anti-HBs(+)were 2 cases(2.00%the study gruoup)and 5 cases(5.00%the control gruop),respectively.Anti-HBsAg(+)and anti-HBc(+)were 2 cases(2.00%the study gruoup)and 1 case(1.00%the control gruop),respectively.Anti-HBsAg(+),anti-HBc(+)and anti-HBeAg(+)were 5 cases(5.00%the study gruoup)and 10 cases(10.00%the control gruop),respectively.Anti-HBsAg(+),anti-HB

关 键 词：乙肝两对半 免疫发光仪 全自动酶免仪 定量研究 

分 类 号：R122.12[医药卫生—环境卫生学]